Potato is one of the most important staple crops in both developed and developing countries of the world. Potato genetic resources are a prerequisite for potato breeding in both conventional and genetic engineering programmes. Cryopreservation has long been considered an ideal means for the long-term conservation of plant genetic resources. This review provides comprehensive information on vari...

Cryopreservation has been recognized as a practical and efficient tool for long-term storage of vegetatively propagated plants. This study was conducted to investigate the effects of sucrose concentration, hardening temperature and different cryopreservation methods on the survival rate of potato shoot tips after cryopreservation. Excised shoot tips of in vitro plantlets of potato cultivars, At...

The present study was aimed to compare the photoautotrophic (PAM) and photomixotrophic micropropagation (PMM) of three potato cultivars (Hermes, Rosetta and Asterix). Cultures were initiated from single node explants and established in MS basal medium with some modifications. Potato plantlets responded in sugar-free media although sugar-containing media (PMM1 and PMM2) performed better as compa...

The use of in vitro potato shoot cultures for protoplast isolation is desirable for consistent protoplast qualities. Ethylene build-up in the culture vessels causes problems for leaf growth (e.g. decreasing leaf surface, dry weight and chlorophyll) in shoot culture. Fifty to one hundred μM Silver thiosulfate (STS) produced a larger leaf area as a source of plant material for protoplast isolatio...

The present investigation was conducted to develop a protocol for rapid plant regeneration in potato. The newly emerge sprout of six potato cultivars were used as explant. Varietal response on in vitro regeneration under different hormonal concentration and combination were studied. MS medium supplemented with 0.5, 1.0, 1.5, 2.0, 4.0 mg L of KIN and IAA in combination of both were employed for ...

The use of in vitro potato shoot cultures for protoplast isolation is desirable for consistent protoplast qualities. Ethylene build-up in the culture vessels causes problems for leaf growth (e.g. decreasing leaf surface, dry weight and chlorophyll) in shoot culture. Fifty to one hundred µM Silver thiosulfate (STS) produced a larger leaf area as a source of plant material for protoplast isolatio...

The use of nitrogen in nutrient media is essential for growth and helps identify highly productive media for potato (Solanum tuberosum L.) micropropagation and adventitious shoot formation. Three potato cultivars (Agria, Marfona and Savalan cultivars) were examined for their growth response with medium containing four different levels of KNO3 (1, 1.25, 1.5 and 1.75 times as much 1.9 g l-1) and ...

In vitro evaluations of salinity (NaCI) effects on three potato cultivars (Atlanta, Shepody and Shilbilaty ) were investigated with five NaCI levels ( 0, 25, 50, 75 and100 mM) by using single node explants. Significant differences were noticed among the cultivars followed by different NaCl levels. Salinity stress gradually depressed plant growth and root development with increased NaCl concentr...