A simple, sensitive, rapid (3 min), and highly reproducible solid-phase assay for the detection of proteins in the low nanogram range (4 ng) is described. The assay is based on differential Ponceau S staining of the protein spots on nitrocellulose and quantification of the protein-dye complexes on lubricated membranes using a densitometer. The dye solution used for protein staining contained 0....

The photodegradation of Ponceau-S dye was investigated using UV radiation in presence of nanosized Fe2 O3 .Removal efficiency of Ponceau-S was sensitive to the operational parameters such as dye concentration, catalyst dose, pH, contact time, TOC and COD. The photocatalytic treatment of red colored Ponceau-S dye by magnetic nano semiconductor (Fe2 O3 )is an effective, economic and faster mode. ...

OBJECTIVES Most clinical laboratories replaced their manual precipitation techniques for the determination of urinary protein with automated dye binding assays or benzethonium chloride-turbidimetric assays. Few studies have validated these assays for the measurement of urinary proteins in the normal range. DESIGN AND METHODS This study compares four automated assays for the measurement of uri...

A sensitive and convenient electrochemical method was developed for the simultaneous determination of the isomers of Ponceau 4R and Amaranth by an expanded graphite paste electrode (EGPE). The EGPE was prepared by mixing EG with solid paraffin. Compared with flake graphite paste and carbon paste electrodes, the EGPE showed higher electrocatalytic activity towards the oxidization of Ponceau 4R a...

Azo dyes are widely used in textile, printing, cosmetic, drug and food-processing industries. They are also used extensively in laboratories as either biological stains or pH indicators. The extent of such use is related to the degree of industrialization. Since intestinal cancer is more common in highly industrialized countries, a possible connection may exist between the increase in the numbe...

Featured Article: Pesce MA, Strande CS. A new micromethod for determination of protein in cerebrospinal fluid and urine. Clin Chem 1973;11:1265–7. This paper described a dye-binding micromethod for measuring protein concentrations in cerebrospinal fluid and urine. The method involved coprecipitation of the proteins with a Ponceau S dye trichloroacetic acid solution, dissolving the protein preci...

Problem statement: Current researches had shown that the photostability of a luminescent solar collector depend on the method of its preparation and type of using dye. This research therefore aimed at studying the stability of luminescent solar collector prepared by sol-gel spin coating method using Ponceau 2R. Approach: The effect of silica matrix on the photostability and fluorescence propert...