The objective of the study was to investigate the antioxidant effect of Chinese medicine Coriolus versicolor polysaccharide on brain tissue and its mechanism in rats. SOD, MDA and GSH-Px levels in rat brain tissues were determined with SD rats as the animal model. The results showed that Coriolus versicolor polysaccharide can reduce the lipid peroxidation level in brain tissues during exhaustiv...

A strain GP32 which produces a highly viscous extracellular polysaccharide was conducted with soil samples and identified as Pseudomonas species. The culture flask conditions for the production of extracellular polysaccharide by Pseudomonas sp. GP32 were investigated. The most suitable carbon and nitrogen source for extracellular polysaccharide production were galactose and (NH4)2SO4. The optim...

In plants, secondary metabolites and polysaccharides interfere with genomic isolation procedures and downstream reactions such as restriction enzyme analysis and gene amplification. The removal of such contaminants needs complicated and time-consuming protocols. In this study, a simple, rapid and efficient method for leaf DNA extraction was optimized. This method use small amount of plant mater...

Nanostructured zinc oxide (ZnO) was successfully synthesized by precipitation method using the extract of Cylea barbata Miers (CBM) leaves as a soft template after 3 h of calcinations in furnace at 500oC in the open air. Characterization results showed that the nanostructured ZnO had hexagonal wurtzite structure with space group P63mc and nano-scale particle diameter. It was suggested that the ...

Pyruvic acid is an immunological determinant of the quite rigorously type-specific capsular polysaccharide of pneumococcal type IV (S IV). Removal of pyruvic acid by mild hydrolysis converts the capsular polysaccharide of type IV into an analog of the pneumococcal group-specific C-substance. Depyruvylated S IV resembles C-substance so closely immunologically that it not only precipitates a high...

Biomimetic scaffolds are desirable for 3D cell culture, yet optically transparent, macroporous, polysaccharide hydrogels have been limited. By incorporating soluble monoand disaccharide additives during cryogelation, we synthesized biomimetic scaffolds and gained insight into the mechanism of their formation. The mono/disaccharide additives affect the size of ice crystal porogens and interact w...

Investigation of the immunologically active substance in Mycobacterium Zeprae has been hampered because of the unavail ability of pure cultures. However, this difficulty can now be circumvented in part by taking advantage of the identification of the group-specific antigen, Polysaccharide I (PolyINb ), present in Nocardia and Mycobacteria ( I. 3 G. 8) . This has been chemically characterized as...

Solubilization of heparan sulfate proteoglycans from a rat liver membrane fraction was obtained by the use of the charged detergent deoxycholate or alternatively a combination of NaCl and the nonionic detergent Triton-X 100. Subsequently, proteoglycans solubilized from microsomal and plasma membrane fractions, respectively, were purified by a procedure involving gel chromatography, anion exchan...

Tubular membrane filtration is an important process when feed waters with a relatively high solids content are filtered. Such solids would normally have to be removed in a pre-treatment stage if spiral wound modules are to be used. High solids content occurs for example in high turbidity surface waters, wastewaters that contain fibrous materials or in waters where coagulants are added. Tubular ...