In the present study, properties of silver nanoparticles (AgNPs) such as average size, size distribution andmorphology were investigated by Tollens, polysaccharide, modified polysaccharide and microbial methods.The synthesized AgNPs were characterized by UV-visible spectroscopy, scanning electron microscopy(SEM), transmission electron microscopy (TEM), dynamics light scatterin...

S.typhimurium is a gram negative bacterium causing serious enteric or extraintestinal infections in human, cattle and birds. These kinds of infections may lead to some permanent or nonpermanent complications like endophthalmitis, Guillain-Barre syndrome and osteomyelitis . Multi-resistant strains of S.typhimurium have been reported from several countries, and these infections are a major public...

Recently, some polysaccharides showed therapeutic potentials for the treatment of neurodegenerative diseases while the most important property, their permeability to the blood brain barrier (BBB) that sheathes the brain and spinal cord, is not yet determined. The determination has been delayed by the difficulty in tracking a target polysaccharide among endogenous polysaccharides in animal. We d...

In order to extract the polysaccharide B of brucella used in Radial Immunodiffusion test (RID) for detection of infected cattle, a quick, simple and safe method is proposed. It consists of using phenol killed cells of B. melitensis 16 M known to produce polysaccharide B. After autoclaving brucella cells resuspended in saline, the cells are separated by centrifugation and the polysaccharide B is...

A type specific polysaccharide has been isolated from the autolyzed broth of Type I Pneumococcus by a modified Avery and Goebel's method. The newly prepared polysaccharide reacts with the homologous immune rabbit serum which has been completely absorbed with the acetyl polysaccharide of Avery and Goebel. The newly prepared polysaccharide produces passive immunity in mice and rats and possibly i...

the role of vi-capsular polysaccharide (vi-cps) in human immunity against infection caused by salmonella typhi is well known. the downstream process of purification generally causes depolymerization of vi-cps to a nonimmunogenic low molecular weight form. in the present study, a standard strain of sal. typhi ty6s was grown under submerge cultural conditions in a pilot-plant scale of 90 liter fe...

The role of Vi-capsular polysaccharide (Vi-CPS) in human immunity against infection caused by Salmonella typhi is well known. The downstream process of purification generally causes depolymerization of Vi-CPS to a nonimmunogenic low molecular weight form. In the present study, a standard strain of Sal. typhi Ty6s was grown under submerge cultural conditions in a pilot-plant scale of 90 Liter fe...

We disagree with the conclusions reached by Bhushan et al. (5) in their study comparing different methods of coating enzyme-linked immunosorbent assay (ELISA) plates for estimation of group B Streptococcus type III (GBS III) polysaccharide-specific antibody concentrations in human serum. We believe that interpretation of the data as suggested in their paper could lead to serious errors in the u...

A hemagglutination method employing tanned human O erythrocytes sensitized with purified Group A streptococcal polysaccharide has been developed to measure A polysaccharide antibody in antistreptococcal rabbit and human sera. The reaction of sensitized RBC with known Group A streptococcal rabbit antisera, inhibition of hemagglutination with A polysaccharide, and abolition of hemagglutination an...