نتایج جستجو برای: Polymerarase Chain Reaction

تعداد نتایج: 627571  

D.O.F. Skibinski S. Rezvani Gilkolaei,

A partial sequence of the mtDNA ND5 gene region was used for population study in Persian sturgeon (west and east areas of southern Caspian Sea). The result showed that although this approach was informative for phylogenetic study in sturgeon, it was less informative for population study in Persian sturgeon. 

Journal: :journal of biotechnology and health sciences 0
taghi naserpour farivar cellular and molecular research center, qazvin university of medical sciences, qazvin, ir iran; cell and molecular research center, qazvin university of medical sciences, qazvin, ir iran. tel: +98-9128801401, fax: +98-2813324971 pouran johari cellular and molecular research center, qazvin university of medical sciences, qazvin, ir iran mohammad hashemi shahri infectious diseases and tropical medicine research center, zahedan university of medical sciences, zahedan, ir iran mohammad naderi infectious diseases and tropical medicine research center, zahedan university of medical sciences, zahedan, ir iran roya alavi infectious diseases and tropical medicine research center, zahedan university of medical sciences, zahedan, ir iran batoul sharifi-mood infectious diseases and tropical medicine research center, zahedan university of medical sciences, zahedan, ir iran

results our study showed that the sensitivity and specificity of taqman real time pcr was 96% and 95% respectively. these values were 89% and 90, 38% and 100%, 6% and 100% for pcr, culture and microscopy, respectively. conclusions our study showed that sensitivity of taqman real time pcr was higher that pcr, culture and microscopy but specificity of culture and microscopy was more than pcr and ...

Journal: :iranian journal of pathology 2015
phiza aggarwal deepak aggarwal

dear editor-in-chief we read with interest the study by khazaei et al. (1) in which the authors have nicely concluded that pcr is more sensitive test than ziehl-neelsen staining and histo-pathological examination for the diagnosis of tuberculosis (tb). they have rightly pointed to use pcr, selectively, in acidfast bacilli negative paucibacillary forms of tb. however, we intend to highlight few ...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه کردستان - دانشکده کشاورزی 1392

بیماری¬های ویروسی ناشی از نپوویروس¬ها از مخرب¬ترین بیماری¬های ویروسی مو در سراسر جهان به حساب می¬آیند که با نماتدهای خانواده longidoridae منتقل می¬شوند. اغلب این ویروس¬ها دارای میزبان¬های طبیعی متعددی در بین گیاهان زینتی و غیر زینتی، درختان میوه و علف¬های هرز می¬باشند. پیکره اعضای این جنس دو بخشی، جورقطر و به قطر حدود 30 نانومتر می¬باشد. غلظت این ویروس¬ها در گیاه و به ویژه تاک پایین است و در نت...

Journal: :jundishapur journal of microbiology 0
abdolhassan kazemi tropical and infectious disease research center, tabriz university of medical sciences, tabriz, ir iran mehraban falahati department of medical parasitology and mycology, tehran university of medical sciences, tehran, ir iran; corresponding author: mehraban falahati, parasitology and mycology department, school of medicine, tehran university of medical sciences, tehran, ir iran . tel.: +98-2188058653, fax: +98-2188058653, e-mail: ashraf hajipoor tropical and infectious disease research center, tabriz university of medical sciences, tabriz, ir iran abbasali jafari department of medical mycology, school of medicine, yazd university of medical sciences, yazd, ir iran mohammad asghar zadeh tropical and infectious disease research center, tabriz university of medical sciences, tabriz, ir iran

results 162 yeast species from 250 specimens were isolated in sabroud dextrose agar (sda) medium. 106 (65.4%) of them were germ tube formation positive, 86 (53%) chlamydoconidium formation positive and 101(62.4%) were pcr positive. yeast cells and mycelia were detected in the isolates on direct microscopic examination. c. albicans accounted for 66.04% of cases and 34% were non-c. albicans speci...

Journal: :journal of comprehensive pediatrics 0
mohammad pedram thalassemia and hemoglobinopathy, research center, ahvaz jundishapur university of medical sciences, ir iran +98-6113743285, [email protected]; thalassemia and hemoglobinopathy, research center, ahvaz jundishapur university of medical sciences, ir iran +98-6113743285, [email protected]سازمان های دیگر: thalassemia and hemoglobinopathy, research center morteza heidari department of pediatrics, golestan hospital, ahvaz jundishapur university of medical sciences, ir iranسازمان اصلی تایید شده: دانشگاه علوم پزشکی جندی شاپور اهواز (ahvaz jundishapur university of medical sciences) bijan keikhaei thalassemia and hemoglobinopathy, research center, ahvaz jundishapur university of medical sciences, ir iran +98-6113743285, [email protected]سازمان اصلی تایید شده: دانشگاه علوم پزشکی جندی شاپور اهواز (ahvaz jundishapur university of medical sciences)سازمان های دیگر: thalassemia and hemoglobinopathy, research center reza azizi malamiri department of pediatrics, golestan hospital, ahvaz jundishapur university of medical sciences, ir iranسازمان اصلی تایید شده: دانشگاه علوم پزشکی جندی شاپور اهواز (ahvaz jundishapur university of medical sciences) behzad poopak department of medical sciences, tehran islamic azad university of medical sciences, ir iranسازمان اصلی تایید شده: دانشگاه علوم پزشکی جندی شاپور اهواز (ahvaz jundishapur university of medical sciences) kiavash fekri department of pediatric, shafa hospital, ahvaz jundishapur university of medical sciences, ir iranسازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (islamic azad university science and research branch)

background neuroblastoma is the most common extracranial malignant solid tumor in children under 5 years, and it is characterized by wide clinical and biological heterogeneity. n-myc oncogene amplification is considered to be one of the most important prognostic factors used to evaluate survival in these patients. objectives the aim of our study was to determine amplification of the n-myc oncog...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه تربیت مدرس - دانشکده علوم زیستی 1393

آنزیم های dna ligase پایدار به حرارت به علت قابلیت های خود از اهمیت بالایی در زیست شناسی مولکولی برخوردارند. در این بین، آنزیم dna ligase از باکتری thermus thermophilus (tth dna ligase) با قابلیت های ویژه ی خود من جمله پایداری بالای حرارتی و دقت بالای عملکرد، می تواند کاربردهای وسیعی را در تکنیک های تشخیصی بیماری های ژنتیکی مانند تکنیک lcr (ligation chain reaction) و mlpa (multiple ligation-dep...

Journal: :jundishapur journal of microbiology 0
than leslie thian lung department of medical microbiology and parasitology, faculty of medicine and health sciences, university putra malaysia, selangor, malaysia chong pei pei department of biomedical sciences, faculty of medicine and health sciences, university putra malaysia, selangor, malaysia ng kee peng department of medical microbiology, faculty of medicine, university of malaya, lumpur, malaysia. seow heng fong department of pathology, faculty of medicine and health sciences, university putra malaysia, selangor, malaysia; department of pathology, faculty of medicine and health sciences, university putra malaysia, seri kembangan, malaysia, malaysia. tel: +60-389464203, fax: +60-389464232

results all eight candida species were successfully detected, identified and quantitated based on the icl gene. a seven-log range of the gene copy number and a minimum detection limit of 103 copies were achieved. conclusions a one-tube absolute quantification real-time pcr that differentiates medically important candida species via individual unique melting temperature was achieved. analytical ...

Journal: :jundishapur journal of microbiology 0
parisa mohammadi department of microbiology, faculty of biological sciences, alzahra university, tehran, ir iran; department of microbiology, faculty of biological sciences, alzahra university, tehran, ir iran. tel: +98-2185692714, fax: +98-2188058912 aida hamidkhani department of biotechnology, faculty of biological sciences, alzahra university, tehran, ir iran ezat asgarani department of biotechnology, faculty of biological sciences, alzahra university, tehran, ir iran

results the results showed that nl1-gc/ls2 primer set could yield species-specific amplicons, which were well distinguished and allowed better species discrimination than that generated by the its3-gc/its4 primer set, in both dgge and ttge profiles. all five candida species were discriminated by dgge and ttge using the nl1-gc/ls2 primer set. conclusions comparison of dgge and ttge profiles obta...

Journal: :jundishapur journal of microbiology 0
alisha akya nosocomial infection research centre, school of medicine, kermanshah university of medical sciences, kermanshah, ir iran; nosocomial infection research centre, school of medicine, kermanshah university of medical sciences, kermanshah, ir iran. tel: +98-8334274618, fax: +98-8334276477 kamal ahmadi msc student of medical microbiology, school of medicine, kermanshah university of medical sciences, kermanshah, ir iran shahram zehtabian school of medicine, kermanshah university of medical sciences, kermanshah, ir iran afsaneh salimi msc student of medical microbiology, school of medicine, kermanshah university of medical sciences, kermanshah, ir iran azam elahi msc student of medical microbiology, school of medicine, kermanshah university of medical sciences, kermanshah, ir iran sayed hamid madani school of medicine, kermanshah university of medical sciences, kermanshah, ir iran

background aseptic meningitis is the most common type of meningitis and is characterized by meningeal inflammation that is not linked to identifiable bacterial pathogens in cerebrospinal fluid (csf). objectives this study aimed to evaluate the frequency of aseptic meningitis caused by herpesviruses, namely herpes simplex types i and ii (hsv-1, hsv-2), epstein-barr virus (ebv), cytomegalovirus (...

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