The effect of aqueous smoke extract, derived from Tanacetum parthenium, onin vitro plantlet regeneration from rapeseed (Brassica napus L. cv. Topas)microspore-derived embryos (MDEs) was evaluated in this study. Inoculation ofrapeseed MDEs with the smoke-extract dilutions (1:250, 1:500, 1:1000 and 1:2000)for various times (5, 10 and 15 min) significantly enhanced the percentage ofregenerated pla...

Here, efficient plantlet regeneration via somatic embryogenesis from Panax quinquefolium hairy roots was reported. Callus development was optimal on MS medium containing 2,4-dichlorophenoxyacetic acid (1 mg l) and -naphthalene acetic acid (1 mg l). After a 4-week growth, calli were transferred onto MS medium with different combinations of plant growth regulators (PGRs). The highest embryogenesi...

background and objectives: bitter melon is one of the anti-cancer medicinal plants that it’s wide level in vitro propagation and anti-cancer material production was necessary. different factors such as genotype, selected explants’ type and it`s preparation conditions influenced regeneration content. plant growth regulator`s effect on callogenesis and indirect regeneration from parangipetai indi...

A protocol was developed for high frequency in vitro multiplication of an ornamental orchid, Phalaenopsis amabilis (L.) Blume var. Jawa, using plant growth regulators (PGRs). Protocorm-like bodies (PLBs), as explants were cultured on Murashige and Skoog (MS) medium fortified with various concentrations of kinetin (KIN) and indole-3-butyric acid (IBA), either individually or in...

Establishment of efficient protocol for the induction of somatic embryogenesis and plantlet regeneration from shoot tip explants was achieved in Glycine max (L).Merr. cv.PK472. Highest frequency (95%) of embryogenic callus formation was obtained from shoot tip explants of Glycine max (L).Merr.cv.PK472 on Murashige and Skoog (MS) medium fortified with different concentration of 2,4-Dichloropheno...

Different explants of Crocus sativus L. such as corm, style, ovary, petal, anther and leaf were cultured on LS and MS media supplemented with NAA and BAP or 2,4-D plus BAP at different combinations. Shoot and corm induction occurred in addition to the formation of other organs. Efficient plantlet regeneration with strong roots occurred at 1 mg 1-I of both NAA and BAP. An increase in 2,4-D l...