Background: Species identification in commercially processed food and feed products is one of the important issues. This study was conducted to develop a genetic method for the detection of pig and cattle species in processed food and feed products using newly designed species-specific primers targeting mitochondrial 12S rRNA gene fragments. Methods: Two sets of specific primers were designed ...

The enterotoxins (ETs) of Clostridium perfringens isolated from enterotoxemic or diarrheic alpacas, pigs, calves, dogs, and horses were obtained from sporulated cell extracts. The ETs from alpaca, pig, and calf isolates were chromatographed on Sephadex G-lOO. Monoclonal antibodies (MAbs) against ETs derived from alpaca, pig, and calf C. perfringens isolates were produced. The MAbs were used...

      Flavonoids could serve as potent inhibitors of xanthine oxidase (XO). In the present study, the effects of Ruta graveolens L. extract and its major isolated flavonoids, quercetin and rutin, on guinea pig liver XO have been investigated. The inhibitory effects of R. graveolens, quercetin and its glycoside form, rutin, were assayed spectrophotometrically. R. graveolens...

Chromosomal patterns of experimental animals are useful tools for cytogenetics research and animal breeding. Chromosome investigations of the hairless guinea pig are rare, therefore, karyotype of hairless guinea pigs (twelve male and female) was studied using metaphase spreads of bone marrows and G banding techniques. The chromosomes diploid number was 2n= 64 and polymorphism of three type chro...

there is documented evidence that infection in laboratory animals can often influence the outcome of experiments. all infections, apparent or inapparent, are likely to increase biological variability. as a research project concerning the diversity and distribution of parasites of rabbit and guinea pig in a conventional laboratory animal house, about 87 rabbits (from 700 ) and 105 guinea pigs (f...

In this research, anatomical descriptions of the structure of the temporal bone and auditory ossicles have been performed based on dissection of ten guinea pigs. The results showed that, in guinea pig temporal bone was similar to other animals and had three parts; squamous, tympanic and petrous .The tympanic part was much better developed and consisted of oval shaped tympanic bulla with many re...

onion (allium cepa) contains high levels of flavonoids. although there are many studies indicating the inhibitory effects of flavonoids on xanthine oxidase, there is no report on the effect of onion on this enzyme. therefore, in the present study, the inhibitory effects of onion on xanthine oxidase are investigated. fresh filtered juice of onion was prepared and its inhibitory effect on guinea ...

in this research, anatomical descriptions of the structure of the temporal bone and auditory ossicles have been performed based on dissection of ten guinea pigs. the results showed that, in guinea pig temporal bone was similar to other animals and had three parts; squamous, tympanic and petrous .the tympanic part was much better developed and consisted of oval shaped tympanic bulla with many re...

in this study, considering the normal ingredients in guinea pig's milk, by using cow milk, mixture of vitamins and minerals, a milk substitute was prepared and sterilized by autoclaving and filtration methods. in food analysis of produced milk, the important substances values, such as protein, fat and ash, were suitable. milk substitute was fed to four groups of newborn's guinea pigs that we...