Carboxypeptidase from Aspergillus saitoi removes acidic, neutral and basic amino acids as well as proline from the C-terminal position at pH 2-5. cpdS, a cDNA encoding A. saitoi carboxypeptidase, was cloned and expressed. Analysis of the 1816-nucleotide sequence revealed a single open reading frame coding for 523 amino acids. When A. saitoi carboxypeptidase cDNA was expressed in yeast cells, ca...

Treatment of wastewater containing carbohydrates by Pichia saitoi growing on beet molasses was investigated in a well-mixed continuous tank as an alternative to bulking control.The yeast strain that used in this work was isolated from non-alcoholic beverage industrial wastewater, with a view on TOC removal compared with other strains in previous study.In this research the isolated yeast showed ...

Rice mold starters prepared from Aspergillus species are commonly used for the manufacture of koji in the production of oriental fermented foods. Methanol extracts of rice mold starters fermented by the Aspergillus species, A. awamori, A. kawachii, A. oryzae, A. saitoi, and A. sojae, were examined for their antioxidative activity by using a 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavengin...

The formation mechanism for the potent antioxidative o-dihydroxyisoflavones, 8-hydroxydaidzein (8-OHD) and 8-hydroxygenistein (8-OHG), was studied by incubating whole soybeans in a solid culture and a soybean extract in a liquid culture with Aspergillus saitoi. Analyses of changes in the isoflavone analogue content, β-glucosidase activity, and isoflavone hydroxylation ability indicated that 8-O...

Five species of the genus Homaloxestis Meyrick are recorded from China. Homaloxestis aciformis sp. nov. from Chongqing and H. ellipsoidea sp. nov. from Yunnan are described as new. Homaloxestis plocamandra (Meyrick, 1907), H. australis Park, 2004 and H. saitoi Park, 2004 are newly recorded from China. Photographs of adults and genital structures are provided. All the known species in China are ...

background: trypsin modulating oostatic factor (tmof) terminates trypsin biosynthesis in adult and larval mos­quito gut. it will inhibit the growth and development of mosquito larvae feeding on it resulting in death by starvation. the objective of this study is to determine the effective dose of pichia-tmof and the combination of pichia-tmof with bacillus thuringiensi s ( bt) as larvicide on ae...

An Aspergillus saitoi protease preparation, Molsin, was found to contain beta-glucosidase as well as protease activities. Application of Molsin to soybean curd improved its functionality by converting the contained isoflavone glycosides to their aglycones through beta-glucosidase, and also modified the rheological property into a creamy consistency through protease. The enzymatically modified s...

Pectinolytic enzymes are greatly important in winemaking due to their ability to degrade pectic polymers from grape, contributing to enhance process efficiency and wine quality. This study aimed to analyze the occurrence of pectinolytic yeasts during spontaneous fermentation of Argentine Bonarda grape, to select yeasts that produce extracellular pectinases and to characterize their pectinolytic...