A highly sensitive and specific TaqMan real-time PCR was used to quantify hepatopancreatic parvovirus (HPV) type III infections in wild broodstocks and hatchery-reared postlarvae (PL) of Fenneropenaeus chinensis. Totals of 159 and 162 wild brooders from three locations were captured, and 140 and 180 PL were obtained from seven and six commercial hatcheries in 2007 and 2008, respectively. Among ...

Four species of penaeid prawn cultured in Australia (Penaeus monodon, Penaeus esculentus, Marsupenaeus japonicus and Fenneropenaeus merguiensis) were injected with a virulent preparation of gill-associated virus (GAV). P. monodon (average weight = 8.9, 13.9 and 19.2 g), P. esculentus (average weight = 19.5 g), F. merguiensis (average weight = 10.5 g), and small (average weight = 5.8 g) M. japon...

A lectin from the hemolymph of the banana shrimp Penaeus (Fenneropenaeus) merguiensis expressed higher agglutination activity against rabbit erythrocytes than those from human, and its activity was Cadependent. The hemagglutinating activity of the hemolymph lectin was stable up to 55 C and optimal at pH 7.5-8.0. N-acetylated sugars, ManNAc, GlcNAc, GalNAc, and NeuNAc, were effective inhibitors ...

This study reports a primer set for amplifying a partial fragment of about 610 bp in the fast mutating mitochondrial control region in shrimps of the genus Penaeus (Decapoda: Penaeidae). The utility of this amplified fragment for studying population differentiation and structuring, compared with more conservative mitochondrial genes (16S rRNA and COI), was explored in P. merguiensis populations...

Upaya untuk meningkatkan pemanfaatan protein dalam pakan dapat dilakukan dengan penambahan bakteri proteolitik. Penelitian ini bertujuan skrining proteolitik pada saluran pencernaan udang Jerbung (Penaeus merguiensis) sebagai kandidat probotik. Skrining mengisolasi dari yang diperoleh tangkapan nelayan di pesisir pantai Pasuruan, Jawa Timur. Dari hasil isolasi lebih 30 koloni tumbuh media, sela...

Translationally controlled tumour proteins (TCTP) cDNA were cloned from the haemolymph of Scylla paramamosain by reverse transcription PCR using primers derived from Penaeus monodon. Nucleotide sequence analysis revealed 507 bp open reading frame (ORF) encoding 168 amino acid residues with a predicted molecular mass and pI of approximately 19.2 kDa and 4.5, respectively. Interestingly, mud crab...

Slow growth caused by viral diseases has become a major constraint in shrimp aquaculture. Laem-Singh virus (LSNV), a positive-sense single-stranded RNA (ssRNA) virus, has been identified in Penaeus monodon showing slow growth syndrome. To examine the host-range and transmission modes of the virus, 6 species of penaeid shrimp of varying life stages, sourced from the wild and from farms, as well ...

We describe a duplex real-time PCR assay using TaqMan probes for the simultaneous detection of monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV). Both MBV and HPV are shrimp enteric viruses that infect intestinal and hepatopancreatic epithelial cells. Both viruses can cause significant mortalities and depressed growth in infected larval, postlarval, and early juvenile stages of sh...

Offshore catches of banana prawns, Penaeus merguiensis, are correlated with the extent of mangrove forests. However, recent evaluation has questioned whether the apparent relationship between juvenile penaeids and mangroves reflects specific utilisation of mangroves or just the use of shallow, organically rich, muddy habitats. We investigated this by focussing on juvenile P. merguiensis within ...