Background: In spite of different methods of treatment, there is not a simple, safe and complete curative treatment for cutaneous Leishmaniasis (CL), yet. Objective: To compare the efficacy of intralesional Glucantime injection alone, with the combined triple therapy of cryotherapy, paramomycin ointment and intralesional Glucantime in the treatment of CL. Materials and methods: 157 patien...

Visceral leishmaniasis is a neglected tropical disease endemic in East Africa where improved patient-adapted treatments are needed. The Leishmaniasis East Africa Platform (LEAP) was created in 2003 to strengthen clinical research capacity, serve as a base for training, and evaluate and facilitate implementation of new treatments. Major infrastructure upgrades and personnel training have been ca...

Background and objectives: The different Aloaceae plant extracts have been tested and found to contain components with anti leishmanial activity. Our study was conducted to assure anti leishmanial activity of the methanol extract of Aloe otallensis on the promastigot stage of Leishmania aethiopica as compared to currently used drugs and also tried to screen its phytochemical constitutes. Method...

Leishmaniases are parasitic diseases occurring in endemic tropical and subtropical areas and caused by protozoa of the genus leishmania, transmitted by a diptera (sand fly). We here report a case of topical cutaneous leishmaniasis discovered in a 15-year old boy with painless ulcer on his left leg, who had been staying in South Africa. Clinical examination showed painless non-itchy ulcer, occur...

Visceral leishmaniasis (VL) or Kala-azar is the most severe form of leishmaniasis and is uniformly fatal, if untreated. An estimated 500,000 new cases occur per year, 90% of which occur in the endemic areas of India, Bangladesh, Sudan Nepal and Brazil. More than 100,000 cases of VL occur in India alone every year and the state of Bihar accounts for more than 90% of these. Over the years new the...

UNLABELLED Cutaneous leishma iasis (CL) is zoonosis with a spectrum of cutaneous manifestations caused by protozoan parasites of thegenus Leishmania.Manifestation varies according to the parasite virulence and the host immune response. Pentavalent antimonials (sodium stibogluconate and meglumine antimoniate) have been used as a first-line therapy for the last 70 years around the world.We report...

Routine production of large numbers of transgenic plants is required to fully exploit advances in cassava biotechnology and support development of improved germplasm for deployment to farmers. This article describes an improved, high-efficiency transformation protocol for recalcitrant cassava cultivar TME14 preferred in Africa. Factors that favor production of friable embryogenic calli (FEC) we...

BACKGROUND Sodium antimony gluconate (SAG) unresponsiveness of Leishmania donovani (Ld) had effectively compromised the chemotherapeutic potential of SAG. 60s ribosomal L23a (60sRL23a), identified as one of the over-expressed protein in different resistant strains of L.donovani as observed with differential proteomics studies indicates towards its possible involvement in SAG resistance in L.don...

Fragments of mitochondrial DNA from Saccharomyces cerevisiae were joined to the Escherichia coli plasmid pBR322 by the poly(dA):poly(dT) tailing method. A total of 347 transformants containing DNA inserts were isolated. The size of the inserted DNA ranges from 0.5 to 5 kilobases. To screen for mitochondrial tRNA genes, we isolated mitochondrial tRNAs, end-labeled them with 32P, and hybridized t...