ABSTRACT Potato field isolates (Solanum tuberosum) of Potato virus Y (PVY) collected from the midwestern and western United States were characterized using serological, molecular, and biological assays. PVY field isolates were grouped into the previously defined categories: PVY(O), European PVY(NTN), North American PVY(NTN), and PVY(N:O) recombinant and four previously undefined groups. Studies...

A multiplex AmpliDet RNA assay was developed for the specific detection of potato virus Y (PVY), and for the differentiation of the PVY(N), PVY(O/C) strains and the tuber necrotic isolates (PVY(NTN)). The assay is based on the generic amplification of a region within the coat protein coding region of all known PVY isolates by nucleic acid sequence-based amplification (NASBA) and strain-specific...

Potato virus Y has emerged as a threatening problem in all potato growing areas around the globe. PVY reduces the yield and quality of potato cultivars. During the last 30 years, significant genetic changes in PVY strains have been observed with an increased incidence associated with crop damage. In the current study, computational approaches were applied to predict Potato derived miRNA targets...

Potato y disease caused by potato virus y (PVY) is widespread wherever potato is grown. PVY affects plants and yields. The effectiveness of extracts from garlic cloves (GE) and onion stems (OE) against potato virus y (PVY) in vitro and in vivo has been evaluated. GE and OE reduced the in vitro and in vivo infectivity of PVY to a certain extent, expressed as the number of local lesions induced b...

PVY is a member of the Potyviridae, the largest plant virus family. For general information on PVY see the Web Site (life.anu.edu.au/viruses/ICTVdB/57010001.htm). Currently, three main strains of PVY are know. (i) PVY (ordinary or common strain) is widely distributed throughout the potato growning areas of the world. This strain induces mild to severe mosaic and can cause stem necrosis in some ...

We show here that the pvr2 locus in pepper, conferring recessive resistance against strains of potato virus Y (PVY), corresponds to a eukaryotic initiation factor 4E (eIF4E) gene. RFLP analysis on the PVY-susceptible and resistant pepper cultivars, using an eIF4E cDNA from tobacco as probe, revealed perfect map co-segregation between a polymorphism in the eIF4E gene and the pvr2 alleles, pvr2(1...

Potato virus Y (PVY) is an important plant pathogen, whose host range includes economically important crops such as potato, tobacco, tomato, and pepper. PVY presents three main strains (PVY(O), PVY(N) and PVY(C)) and several recombinant forms. PVY has a worldwide distribution, yet the mechanisms that promote and maintain its population structure and genetic diversity are still unclear. In this ...

In this study, the full-length nucleotide sequences of four Iranian PVY isolates belonging to PVY(N) strain were determined. The genome of Iranian PVY isolates were 9,703-9,707 nucleotides long encoding all potyviral cistrons including P1, HC-Pro, P3, 6K1, CI, 6K2, VPg, NIa-Pro, NIb and CP with coding regions of 825, 1,395, 1,095, 156, 1,902, 156, 564, 732, 1,557 and 801 nucleotides in length, ...

In this study, the recovery phenomenon following infection with Potato virus Y (PVY) was investigated in tobacco (Nicotiana tobaccum), tomato (Solanum lycopersicum) and potato (Solanum tuberosum) plants. In tobacco plants, infection of severe strains of PVY (PVYN or PVYN:O) induced conspicuous vein clearing and leaf deformation in the first three leaves above the inoculated leaves, but much mil...

Potato virus Y (PVY) is the type member of the potyvirus group that is the largest of the plant virus groups. Among several PVY strains, PVY° strains are spread worldwide (1) and PVY-O is one of Japanese PVY° strains. We propagated PVY-0 on Nicotiana tabacum cv. Samsun NN and isolated its genomic RNA. The synthesized double-stranded cDNA was inserted into the EcoKl site of pBR322 using £coRI li...