نتایج جستجو برای: PVX

تعداد نتایج: 224  

Journal: :Virology 1972
J H Tremaine H O Agrawal

The effect of method of purification and incubation with trypsin on the amino acid composition and molecular weight of potato virus X (PVX) protein was determined. PVX wae prepared by four methods, two of which included incubation of unclarified plant sap at room temperature for 24 hr before addition of organic solvents. The molecular weights of the proteins of PVX determined by SDS polyacrylam...

Journal: :Phytopathology 2006
A A Bazzini H E Hopp R N Beachy S Asurmendi

ABSTRACT The expression of a gene that encodes coat protein (CP) of Potato virus X (PVX) in transgenic tobacco plants confers a high level of CP-mediated rresistance (CP-MR) against PVX infection. To determine if posttranscriptional gene silencing (PTGS) plays a role in resistance, transgenic plants expressing PVX CP were challenged against PVX under conditions in which PTGS was suppressed by l...

2016
Hoseong Choi Won Kyong Cho Kook-Hyung Kim

Because viruses encode only a small number of proteins, all steps of virus infection rely on specific interactions between viruses and hosts. We previously screened several Nicotiana benthamiana (Nb) proteins that interact with the stem-loop 1 (SL1) RNA structure located at the 5' end of the potato virus X (PVX) genome. In this study, we characterized two of these proteins (NbCPIP2a and NbCPIP2...

Journal: :The EMBO journal 1997
S M Angell D C Baulcombe

Tobacco plants were transformed with constructs in which the transgene was a cDNA of replicating potato virus X (PVX) RNA. The constructs, referred to here as amplicons, were the intact genome of PVX and PVX constructs modified to carry the beta-glucuronidase (GUS) reporter gene either as an additional gene or as a replacement for the coat protein gene (PVX/GUS/CP and PVX/GUS respectively). Tra...

2015
Christina Dickmeis Mareike Michaela Antonia Honickel Rainer Fischer Ulrich Commandeur

We have generated hybrid chimeric potato virus X (PVX) particles by coexpression of different PVX coat protein fusions utilizing tobacco mosaic virus (TMV) and PVX-based expression vectors. Coinfection was achieved with a modified PVX overcoat vector displaying a fluorescent protein and a TMV vector expressing another PVX fluorescent overcoat fusion protein. Coexpression of the PVX-CP fusions i...

Journal: :Virus research 2014
Denis Kutnjak Rocio Silvestre Wilmer Cuellar Wilmer Perez Giovanna Müller Maja Ravnikar Jan Kreuze

Potato virus X (PVX; genus Potexvirus, family Alphaflexiviridae, order Tymovirales) is one of the most widespread and intensively studied viruses of potato. However, little is known about its diversity in its likely center of radiation, the Andean region of South America. To fill this gap, the strategy of Illumina deep sequencing of small RNAs was used to obtain complete or near complete genome...

Journal: :The Plant cell 1992
C. J. Braun C. L. Hemenway

The first open reading frame (ORF 1) of potato virus X (PVX) encodes a putative replicase gene. Transgenic tobacco lines expressing ORF 1 are resistant to PVX infection when inoculated with either PVX or PVX RNA. Analyses of lines containing various portions of the ORF 1 gene demonstrated that resistance is conferred to plants by expressing approximately the first half of the ORF 1 gene. One li...

Journal: :Virology 2000
O N Fedorkin A Merits J Lucchesi A G Solovyev M Saarma S Y Morozov K Mäkinen

The cell-to-cell movement of the GUS-tagged potato virus X (PVX) coat protein (CP) movement-deficient mutant was restored by potyviral CPs of potato virus A (PVA) and potato virus Y (PVY) in Nicotiana benthamiana leaves in transient cobombardment experiments. Viral cell-to-cell movement of PVX CP mutant was complemented in Nicotiana tabacum cv. SR1 transgenic plants expressing PVY CP: PVX RNA a...

Journal: :Journal of virology 1998
X Ares G Calamante S Cabral J Lodge P Hemenway R N Beachy A Mentaberry

The p24 protein, one of the three proteins implicated in local movement of potato virus X (PVX), was expressed in transgenic tobacco plants (Nicotiana tabacum Xanthi D8 NN). Plants with the highest level of p24 accumulation exhibited a stunted and slightly chlorotic phenotype. These transgenic plants facilitate the cell-to-cell movement of a mutant of PVX that contained a frameshift mutation in...

Journal: :The Journal of general virology 2007
Bong-Nam Chung Tomas Canto Peter Palukaitis

The stability of hybrid plant viruses that might arise by recombination in transgenic plants was examined using hybrid viruses derived from the viral expression vectors potato virus X (PVX) and tobacco rattle virus (TRV). The potato virus Y (PVY) NIb and HCPro open reading frames (ORFs) were introduced into PVX to generate PVX-NIb and PVX-HCPro, while the PVY NIb ORF was introduced into a vecto...

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