BACKGROUND Peroxisome proliferator-activated receptor alpha (PPARA) is a major regulator of fatty acid oxidation and severe hepatic steatosis occurs during acute fasting in Ppara-null mice. Thus, PPARA is considered an important mediator of the fasting response; however, its role in other fasting regiments such as every-other-day fasting (EODF) has not been investigated. METHODS Mice were pre...

To determine the impact of the species difference between rodents and humans in response to peroxisome proliferators (PPs) mediated by peroxisome proliferator–activated receptor (PPAR)a, PPARa-humanized transgenic mice were generated using a P1 phage artificial chromosome (PAC) genomic clone bred onto a ppara-null mouse background, designated hPPARa. In hPPARa mice, the human PPARa gene is expr...

Activation of peroxisome proliferator-activated receptor alpha (PPARα) occurs in animal models of diabetes (DM) and is implicated in pathological responses to myocardial ischemia. Using bioinformatics, we identified a single nucleotide polymorphism (SNP) in the PPARα gene promoter (PPARA -54,642 G>A; rs135561) that altered the consensus sequence for a nuclear receptor binding site. Electrophore...

P eroxisome proliferator activated receptor alpha (PPARa), a ligand inducible transcription factor of the nuclear receptor family, plays an important part in the regulation of many genes involved in lipid metabolism and atherogenesis. This nuclear receptor is the cellular target of the fibrate drugs, which can lower triglyceride levels and reduce risk of acute coronary ischaemic events. 5 Gende...

Peroxisome proliferator-activated receptors (PPARs) are liganddependent transcription factors that activate target genes involved in lipid metabolism, energy homeostasis, and cell differentiation in response todiverse compounds, includingenvironmental chemicals. The liver-expressed receptor PPARa mediates peroxisome proliferative responses associated with rodent hepatocarcinogenesis. Previous s...

RATIONALE Fatty acid oxidation is transcriptionally regulated by peroxisome proliferator-activated receptor (PPAR)α and under normal conditions accounts for 70% of cardiac ATP content. Reduced Ppara expression during sepsis and heart failure leads to reduced fatty acid oxidation and myocardial energy deficiency. Many of the transcriptional regulators of Ppara are unknown. OBJECTIVE To determi...

Background—Adhesion molecule expression on the endothelial cell (EC) surface is critical for leukocyte recruitment to atherosclerotic lesions. Better understanding of transcriptional regulation of adhesion molecules in ECs may provide important insight into plaque formation. Peroxisome proliferator–activated receptor-a (PPARa), a member of the nuclear receptor family, regulates gene expression ...

Aims Our goal was to analyse whether truncated peroxisome proliferator-activated receptor a (PPARa) overexpression induces apoptosis of cardiomyocytes. Methods and resultsWe constructed a recombinant vector of human truncated PPARa and a mammalian expression vector to transfect PPARa into a line of murine cardiomyocytes designated HL-1. Four hallmarks of apoptosis were measured in these transfe...

Citation: Qiu F, Matlock G, Chen Q, et al. Therapeutic effects of PPARa agonist on ocular neovascularization in models recapitulating neovascular agerelated macular degeneration. Invest Ophthalmol Vis Sci. 2017;58:5065– 5075. DOI:10.1167/iovs.17-22091 PURPOSE. This study was designed to evaluate effects of fenofibric acid (Feno-FA), a peroxisome proliferator–activated receptor-alpha (PPARa) ago...

Human cytochrome P450 (CYP) 2C enzymesmetabolize∼30%of clinically prescribed drugs and various environmental chemicals. CYP2C8, an important member of this subfamily, metabolizes the anticancer drug paclitaxel, certain antidiabetic drugs, and endogenous substrates, including arachidonic acid, to physiologically active epoxyeicosatrienoic acids. Previous studies from our laboratory showed that m...