Phosphorodiamidate morpholino oligomers (PMO) are neutrally charged, sequence-specific antisense agents that interfere with targeted gene expression. PMO have been shown to be highly specific and potent therapies after cellular uptake, yet methods to detect PMO in tissue and inside the cell are limited. We offer in this report novel methods for the detection of cellular resident PMO using flow ...

B2 Lack of experienced project managers and PMO leadership Many PMO initiatives do not have experienced project managers and leaders who understand the challenges of a PMO implementation and who are aware of the political terrain in the organization. B3 Lack of dedicated resources in the PMO implementation team In most PMO projects, the team members are shared resources, often pulled into other...

We have recently reported that cell-penetrating peptides (CPPs) and novel chimeric peptides containing CPP (referred as B peptide) and muscle-targeting peptide (referred as MSP) motifs significantly improve the systemic exon-skipping activity of morpholino phosphorodiamidate oligomers (PMOs) in dystrophin-deficient mdx mice. In the present study, the general mechanistic significance of the chim...

A series of amphiphilic peptides modified PMO (Pt-PMO) were prepared, and their antisense effect and toxicity were evaluated both in vitro and in mdx mice. The results showed that the exon-skipping performance of Pt-PMO are relative to the structure of the conjugated peptide: the Pt3/Pt4 composed of six/seven arginines and one myristoylation modified PMO showed more efficacy and with less toxic...

OBJECTIVES Antisense phosphorodiamidate morpholino oligomers (PMOs) are synthetic DNA mimics that specifically inhibit gene expression in pure cultures of Escherichia coli. Previously, an 11 base PMO targeted to an essential gene (acpP) for phospholipid biosynthesis was shown to inhibit growth of a pure culture of E. coli AS19, which has an abnormally permeable outer membrane. The objectives of...

Given the popularity of PMO and international projects, and the difficulties experienced by PMO, it is important and necessary to study PMO in a global context. This paper raises a challenge for a PMO who uses traditional approaches to supervise international projects characterized with external embeddedness. Would such a character demand this PMO be operated differently to adapt to environment...

We investigated a series of Tween 85 modified low molecular weight polyethylenimine (LPEI, 0.8k/1.2k/2.0k)-copolymers (Zs) through simple formulation and covalent conjugation with phosphorodiamidate morpholino oligomer (PMO) for their potential to enhance delivery in vitro and in dystrophic mdx mice. Z polymers significantly enhanced PMO-induced exon-skipping in a GFP reporter-based cell cultur...

Purified menhaden oil (PMO) was characterized and microencapsulated. Baby foods containing PMO and microencapsulated purified menhaden oil (MPMO) were evaluated and compared. PMO had higher total omega-3, total saturated, total monounsaturated, DHA, and EPA contents than MPMO. Emulsion containing PMO (EPMO) exhibited viscoelastic characteristic and droplet size of EPMO was around 2e10 mm. The D...

BACKGROUND Only a minority of patients at high risk for osteoporotic fracture receive treatment. OBJECTIVE Study patients' and physicians' views regarding postmenopausal osteoporosis (PMO) to identify impediments to good care. METHODS A qualitative study involving 18 physicians and 37 women (age 57-87) with PMO. RESULTS All women interviewed considered PMO to be somewhat normal wear-and-t...

UNLABELLED BACKGROUND Recent studies demonstrate that enzymes from the glycosyl hydrolase family 61 (GH61) show lytic polysaccharide monooxygenase (PMO) activity. Together with cellobiose dehydrogenase (CDH) an enzymatic system capable of oxidative cellulose cleavage is formed, which increases the efficiency of cellulases and put PMOs at focus of biofuel research. Large amounts of purified P...