This review focuses on phytase functionality in the digestive tract of farmed non-ruminant animals and the factors influencing in vivo phytase enzyme activity. In pigs, feed phytase is mainly active in the stomach and upper part of the small intestine, and added phytase activity is not recovered in the ileum. In poultry, feed phytase activities are mainly found in the upper part of the digestiv...

The rate of phytate P removal from feed (level of dephosphorylation, DL) and the extent to which the molecule of phytic acid is deprived of phosphate moieties (conversion degree, CD) were studied in vitro and in a feeding trial with broilers fed corn-soybean diets. In the in vitro model, phytase A asymptotically increased DL and CD. Phytase B influenced DL only at low dosages of phytase A [0 or...

Phytase and phytase gene from Aspergillus ficuum (A. ficuum) were used in this study. The results showed that phytase activity reached the peak of 0.17 U/g after 4 d incubation in solid medium for A. ficuum; the optimum pH and temperature of phytase were 2.5 and 50 o C, respectively. A 1.4-kb DNA containing the coding region of phytase gene was isolated and inserted into the expression vectors ...

This study was to assess effects of the pretreatment in allplant based diets with microbial phytase on phosphorous utilization and growth performance of Nile tilapia (Oreochromis niloticus). Pretreatment trials were conducted using phytase at graded doses to determine the optimal dose of phytase. Available phosphorus (P) levels increased significantly with the increased doses of phytase and the...

Response surface methodology (RSM) was used to optimize the cultivation conditions for the production of phytase by recombinant Escherichia coli DH5α. The optimum predicted cultivation conditions for phytase production were at 3 hours seed age, a 2.5% inoculum level, an L-arabinose concentration of 0.20%, a cell concentration of 0.3 (as measured at 600 nm) and 17 hours post-induction time with ...

Role of disulfide bridges in phytase's unfolding-refolding was probed using dynamic light scattering. Phytase was unfolded by guanidinium chloride and then refolded by removing the denaturant by dialysis. Thiol reagents prevented refolding; thus, disulfide bridge formation is an integral step in phytase folding. Catalytic demise of phytase after unfolding and refolding in presence of Tris(2-car...

A study was conducted to investigate the effects of thermostable phytase supplementation on the growth performance and nutrient digestibility of broilers for 42 d. 1-day-old Arbor Acres chicks with similar initial body weight were randomly divided into 3 treatments consisting 6 pens of 20 chicks per pen. The control group was given maize-soybean meal basal diet and the other two groups were fed...

Phytase expressed and anchored on the cell surface of Pichia pastoris avoids the expensive and time-consuming steps of protein purification and separation. Furthermore, yeast cells with anchored phytase can be used as a whole-cell biocatalyst. In this study, the phytase gene of Citrobacter amalonaticus was fused with the Pichia pastoris glycosylphosphatidylinositol (GPI)-anchored glycoprotein h...

Two studies were conducted to determine the true P digestibility (TPD) of black-eyed pea (BEP) and peanut flour (PNF) and the TPD response to phytase supplementation using the regression method. Sequential diets containing 115, 230, or 345 g of BEP/kg (experiment 1) and 115, 230, or 345 g of PNF/kg (experiment 2) without or with 1,000 units of phytase/kg were formulated. Chromic oxide was added...