OBJECTIVE Expression of Spam1/PH20 and its modulation of high/low molecular weight hyaluronan substrate have been proposed to play an important role in murine oligodendrocyte precursor cell (OPC) maturation in vitro and in normal and demyelinated central nervous system (CNS). We reexamined this using highly purified PH20. METHODS Steady-state expression of mRNA in OPCs was evaluated by quanti...

OBJECTIVE Although the spectrum of white matter injury (WMI) in preterm infants is shifting from cystic necrotic lesions to milder forms, the factors that contribute to this changing spectrum are unclear. We hypothesized that recurrent hypoxia-ischemia (rHI) will exacerbate the spectrum of WMI defined by markers of inflammation and molecules related to the extracellular matrix (hyaluronan (HA) ...

Comment on: PH20 is not expressed in murine CNS and oligodendrocyte precursor cells Larry S. Sherman* & Stephen A. Back Division of Neuroscience, Oregon National Primate Research Center, Beaverton, Oregon Department of Cell, Developmental and Cancer Biology, Oregon Health & Science University, Portland, Oregon Department of Pediatrics, Oregon Health & Science University, Portland, Oregon Depart...

M. K. LAURENSON*†, R. A. NORMAN‡, L. GILBERT§, H. W. REID¶ and P. J. HUDSON†§ * CTVM, University of Edinburgh, Easter Bush, Roslin, Midlothian, EH25 9RG, UK; † The Game Conservancy Trust, Upland Research Group, Crubenmore Lodge, Newtonmore, PH20 1BE, UK; ‡ Department of Computing Science and Mathematics, University of Stirling, Stirling, FK9 4LA, UK; § Institute of Biology, University of Stirli...

M. K. LAURENSON*†‡, R. A. NORMAN§, L. GILBERT†, H. W. REID¶ and P. J. HUDSON†‡ * CTVM, University of Edinburgh, Easter Bush, Roslin, Midlothian, EH25 9RG, UK, † Institute of Biology, University of Stirling, Stirling FK9 4LA, UK, ‡ The Game Conservancy Trust, Upland Research Group, Crubenmore Lodge, Newtonmore, PH20 1BE, UK, § Department of Computing Science and Mathematics, University of Stirli...

Protein alterations of turkey breast muscles (Pectoralis major) were investigated at 20 min and 24 h post mortem. Specific activities, quantities and kinetic parameters of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and aldolase A were also determined at 20 min post mortem. Based on the pH values at 20 min post mortem, two groups of samples were classified as rapid glycolysis group (RG; pH...

Background: The hyaluronidases are the enzymes hydrolyze β-1, 4 glycosidic linkage of hyaluronan. Hyaluronan is a polymer consisting of a repeating disaccharide unit found in cumulus ovuforus complex, semen liquid and other tissue. Addition to hydrolyzing the hyaluronan, hyaluronidase can penetrate through the cumulus cells layer that surrounds the oocyte, thus it terms spreading factor. Moreov...

OBJECTIVE To determine whether levels of PH-20, a hyaluronidase similar to that found in human sperm, are elevated in laryngeal cancer tissue. DESIGN In this case-control study. reverse transcription polymerase chain reaction was used to measure levels of PH-20 messenger RNA in tissue taken from laryngectomy specimens. SETTING A university medical center. PATIENTS We compared tissue sampl...