Amongst the various species of Aspergillus section Nigri; Aspergillus luchuensis, associated with food and beverage fermentation in East Asia, is evaluated by multiple researchers as nonochratoxigenic based on the extrolite analysis. Beyond extrolite analysis, in the present study, A. luchuensis strain was analyzed by a rapid Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (P...

background: giardia has the ability to infect many mammals including dogs, cats, deer, mice, ground squirrels, chinchillas, swine, rabbits, pocket mice, oxen, guinea pigs, and humans. giardia lamblia (also giardia duodenalis, g.intestinalis) isolates have been variably divided into two or three genotypes by different investigators, and each group can be divided into subgroups. objectives: we ha...

The study was conducted to find out polymorphism at various growth hormone (bGH) and growth hormone receptor loci (bGHR) by using PCRRFLP technique. Fifty two blood samples of Mehsana buffalo were collected from Livestock Research Station, Sardarkrushinagar Agricultural University, Gujarat. The DNA samples were subjected to PCR amplification using bGH and bGHR specific primers. The PCR products...

Methods Screening for obesity using CDC 2000 criteria was done in 2121 female adolescents aged 13-14 years old in Yogyakarta. BMI > 95 percentile was considered as obese. Among the obese subjects, 78 agreed to be enrolled for this study. HOMA-IR > 3.16 was used to determine the insulin resistance status. DNA was isolated from peripheral blood and UCP2 -866 G/A, UCP3 -55C/T, GHSR1a rs2922126, GH...

Investigation was conducted on 219 Jersey cows. LEPR genotypes were established with the PCRRFLP using the TaqI restriction endonuclease. The frequencies of the threonine encoding C allele and the methionine encoding T allele were 0.79 and 0.21, respectively. The across-families analysis did not revealed any significant effect of the LEPR T945M polymorphism on milk, fat and protein yields. The ...

A 23-year-old married woman presented with primary amenorrhoea well-developed 2ndary sex characteristics. She is chromosomally competent female (46xx) average body built and had no problem in marital relationship. Clinical examinations all relevant investigations including transvaginal ultrasound(TVS) showed uterus, adnexa normal size volume. Progesterone challenge test was negative on 3 occasi...

Source and Description of Primers. The initial primers for the PCR were designed based on human DNA sequence (accession no. X92412; Kolmerer et al., 1996). The position of the forward and reverse primers corresponded to exon 3 and exon 5, respectively. These primers are expected to amplify a fragment of 1.93 kb from human DNA. A PCR fragment of 2.1 kb was amplified from porcine genomic DNA. Par...

The effective management of cyst nematodes requires accurate assessments of the species composition and size of field populations. Compared to traditional diagnostics the polymerase chain reaction offers high specificity, sensitivity and a more rapid means of identifying large numbers of cyst nematode samples. PCRRFLP of the ITS I ribosomal DNA region from Heterodera and Globodera species produ...