A repetitive-extragenic palindromic PCR (rep-PCR) subtyping method (DiversiLab) in conjunction with ribotyping, toxinotyping and antimicrobial-susceptibility testing was used to detect subtypes within Clostridium difficile ribotypes 027 and 078. Clinical isolates of ribotypes 027 (toxinotype III) (n = 30) and 078 (toxinotype V) (n = 23) were provided by health-care facilities across the Republi...

The increased prevalence of Clostridium difficile infection (CDI) has coincided with enhanced transmissibility and severity of disease, which is often linked to two distinct clonal lineages designated PCR-ribotype 027 and 017 responsible for CDI outbreaks in the USA, Europe and Asia. We assessed sporulation and susceptibility of three PCR-ribotypes; 012, 017 and 027 to four classes of disinfect...

BACKGROUND The rate of infection with Clostridium difficile colitis and its associated mortality have been increasing in the last decade. The molecular epidemiology of C. difficile in Israel has not been studied. OBJECTIVES To screen for the existence of the 027 and 078 ribotypes and determine the longitudinal molecular epidemiology of the circulating clinical C. difficile isolates in a large...

the epidemic PCR-ribotype 001. In particular, the identity between the 2 PCR ribotypes was 100% for the HMW proteins and 77% for the LMW proteins. This study provides convincing evidence that the S-layer is well conserved in C. diffi cile PCR-ribotype 027 strains and has high identity with the slpA of the epidemic PCR-ribotype 001. Because C. diffi cile PCRribotypes 027 and 001 are the most fre...

Of 40 ready-to-eat salads, 3 (7.5%) were positive for Clostridium difficile by PCR. Two isolates were PCR ribotype 017 (toxin A-, B+), and 1 was PCR ribotype 001. Isolates were susceptible to vancomycin and metronidazole but variably resistant to other antimicrobial drugs. Ready-to-eat salads may be potential sources for virulent C. difficile.

We have developed a Clostridium difficile PCR ribotyping method based on capillary gel electrophoresis and have compared it with conventional PCR ribotyping. A total of 146 C. difficile isolates were studied: five isolates were reference strains (PCR ribotypes 001, 014, 017, 027 and 053); 141 were clinical isolates comprising 39 Austrian PCR ribotypes collected in the period 2006-2007 at 25 Aus...

pasteurella multocida is considered to be an important cause of ovine pneumonia and results causes considerable economic losses in iran. ribotyping pcr (ribo-pcr) was used for investigating the diversity of ovine and caprine p.multocida. a total of 120 swab tonsil and nasal samples were obtained from sheep and goats. they were analyzed by biochemical tests and ribo-pcr 16s-23s ribosomal rna gen...

Clostridium difficile is the most common cause of antibiotic-associated diarrhoea worldwide. Over the past 10 years, the incidence and severity of disease have increased in North America and Europe due to the emergence of a hypervirulent clone designated PCR ribotype 027. In this study, we sought to identify phenotypic differences among a collection of 26 presumed PCR ribotype 027 strains from ...

OBJECTIVES Therapeutic options in Clostridium difficile infection (CDI) are limited. We examined linezolid activity in vitro and potential therapeutic efficacy using a gut model of CDI. METHODS MICs were determined by agar incorporation for 118 diverse C. difficile faecal isolates, including epidemic strains and strains with reduced susceptibility to metronidazole. CDI was established in two ...

1. Abstract 2. Introduction 3. Phenotypic techniques 3.1. Serotype analysis 3.2. Biotype and phage type analysis 3.3. Antibiotic susceptibility patterns 4. Genotypic techniques 4.1. Plasmid analysis 4.2. Chromosomal restriction fragment analysis and pulsed-field gel electrophoresis (PFGE) 4.3. Chromosomal restriction-hybridization techniques 4.3.1. Ribotype analysis 4.3.2. IS200 4.4. Locus-spec...