BACKGROUND Mucosal epithelia, including those of the oviduct, secrete antimicrobial innate immune molecules (AIIMS). These have bactericidal/bacteriostatic functions against a variety of pathogens. Among the AIIMs, sheep β-defensin-1 (SBD-1) is one of the most potent. Even though the SBD-1 is an important AIIM and it is regulated closely by estrogenic hormone, the regulation mechanism of 17β-es...

background: the basal medium that supports isolated mouse oviduct (imo) is important for supporting embryo development and quality. methods: the culture of ovine ivm/ivf zygotes was done in imo using sofaacibsa and sofaabsa as basal medium of imo and in sofaabsa alone as control. for preparation of imo mature inbred strain c57bl/6 female mice were synchronized and mated with vasectomized males....

BACKGROUND The basal medium that supports Isolated Mouse Oviduct (IMO) is important for supporting embryo development and quality. METHODS The culture of ovine IVM/IVF zygotes was done in IMO using SOFaaciBSA and SOFaaBSA as basal medium of IMO and in SOFaaBSA alone as control. For preparation of IMO mature inbred strain C57BL/6 female mice were synchronized and mated with vasectomized males....

The mammalian oviduct has long been recognized as an organ essential for successful reproduction. Bovine, ovine, porcine, and equine animal models have offered clear advantages for oviduct study related to gamete physiology, fertilization, and early embryonic development. Livestock species are amenable to surgical alteration of the reproductive tract, estrous cycle manipulation, gamete cryopres...

The mammalian oviduct has long been recognized as an organ essential to the success of reproduction. Bovine, ovine, porcine and equine animal models have offered clear advantages for oviduct study related to gamete physiology, fertilization and early embryo development. Livestock species are amenable to surgical alteration of the reproductive tract, estrous cycle manipulation, gamete cryopreser...

Techniques for in vitro production (IVP) of viable embryos have been thoroughly developed in several domestic species in view to improve breeding efficiency. When applied to wild life, these techniques may also help the maintenance of biodiversity through amplification of sparse animals offspring and facilitation of genetic material exchange. During the successive steps of IVP, i.e. oocyte in v...

Sex determination of in-vitro matured/in-vitro fertilized ovine embryos cultured in synthetic oviduct fluid (SOF) medium was performed by the polymerase chain reaction amplification of specific Y DNA sequences so as to test the influence of sex on developmental growth during the preimplantation period. At 144 h post-insemination, embryos with a blastocoel were classified as the fast-developing ...