background: the basal medium that supports isolated mouse oviduct (imo) is important for supporting embryo development and quality. methods: the culture of ovine ivm/ivf zygotes was done in imo using sofaacibsa and sofaabsa as basal medium of imo and in sofaabsa alone as control. for preparation of imo mature inbred strain c57bl/6 female mice were synchronized and mated with vasectomized males....

BACKGROUND The basal medium that supports Isolated Mouse Oviduct (IMO) is important for supporting embryo development and quality. METHODS The culture of ovine IVM/IVF zygotes was done in IMO using SOFaaciBSA and SOFaaBSA as basal medium of IMO and in SOFaaBSA alone as control. For preparation of IMO mature inbred strain C57BL/6 female mice were synchronized and mated with vasectomized males....

Background: The developmental competence of oocytes matured in vitro (IVM) is yet far below than in vivo counterparts. Recent studies suggest that the asynchrony between nuclear/cytoplasmic maturation and the initial low/heterogeneous quality of oocytes are the most important factors affecting IVM success. We investigated whether selection of growing oocytes (based on their glucose 6- phosphate...

Artificial insemination (AI) and embryo transfer (ET) play an important role in the genetic improvement of sheep herds. This review primarily describes the methods that have recently been researched and/or adapted to circumvent the problematic anatomy of the ovine cervix, which prevents the widespread application of transcervical AI and ET in this species. A mechanism of cervical ripening at pa...

Background: Fibroblast growth factors are a great family of growth factors that exert variety of functions in vertebrates including cell growth, survival, differentiation and migration. FGFRs are transmembrane tyrosine kinase receptors transmitting FGF signals. Four types of FGFRs (1/2/3/4) exist in vertebrates. FGF2 is one of the FGFs detected in bovine and ovine uterus flushes, supposing that...

Background: Heat shock may affect different aspects of oocyte maturation and its subsequent development to the blastocyst stage. A series of in vitro experiments was performed to determine whether physiologically heat shock (41°C) disrupts the progression of the ovine oocytes through meiosis, activation and blastocyst formation. Materials and Methods: The cumulus-oocyte complexes (COCs) were as...

Background: This study was designed to respond to the controversy regarding the necessity of oocyte activation after the ICSI procedure for normal embryo development in sheep. Materials and Methods: The effect of chemical stimulation with either 5mM ionomycin (I) for 5 minutes or ionomycin + 2 mM 6-dimethylaminopurine (6-DMAP) for 3 hours on the efficiency of ICSI, was compared in six experimen...

Aim The study aimed to assess the nature of animal origin Staphylococcus aureus strains. The study has zoonotic importance and aimed to compare virulence between two different hosts, i.e., bovine and ovine origin. Materials and Methods Conventional polymerase chain reaction-based methods used for the characterization of S. aureus strains and chick embryo model employed for the assessment of v...

Physical removal of mammalian cumulus-oocyte complexes (COCs) from ovarian follicles results in spontaneous resumption of meiosis, largely because of a decrease in cAMP concentrations, causing asynchrony between cytoplasmic and nuclear maturation and decreased oocyte developmental competence. The aim of this study was to modulate cAMP concentrations within ovine COCs to delay spontaneous nuclea...

Bovine and ovine embryos exposed to a variety of unusual environments prior to the blastocyst stage have resulted in the development of unusually large offspring which can also exhibit a number of organ defects. In these animals, the increased incidence of difficult parturition and of fetal and neonatal losses has limited the large-scale use of in vitro embryo production technologies commonly u...