Saenger sequencing has led the advances in molecular biology, while faster and cheaper next generation technologies are urgently needed. A newer approach exploits nanopores, natural or solid-state, set in an electrical field, and obtains base sequence information from current variations due to the passage of a ssDNA molecule through the pore. A hurdle in this approach is the fact that the four ...

Osmium tetroxide 2,2'-bipyridine (OsBp) is known to react with pyrimidines in ssDNA and preferentially label deoxythymine (T) over deoxycytosine (C). The product, osmylated DNA, was proposed as a surrogate for nanopore-based DNA sequencing due to OsBp's "perfect" label attributes. Osmylated deoxyoligos translocate unassisted and measurably slow via sub-2 nm SiN solid-state nanopores, as well as...

Nanopores are a promising platform in next generation DNA sequencing. In this platform, an individual DNA strand is threaded into nanopore using an electric field, and enzyme-based ratcheting is used to move the strand through the detector. During this process the residual ion current through the pore is measured, which exhibits unique levels for different base combinations inside the pore. Whi...

Treatment of C60 with osmium tetroxide and pyridine gives the osmate ester (2:1 adduct) establishing that oxygen functionality can be added to C60 without disrupting the carbon framework.

catalytic osmylation of cis and trans 2-cyclohexene-cyclohexanol (1a) and (1b) are carried out in the presence of n-methyl morpholine-n-oxide. under the same conditions, only the cis isomer reacted, leading to triol (3). the steric hindrance of the equatorial oh group probably prevents the trans isomer from entering into such reaction. triol (3) was also obtained from the reduction of the diol-...

Catalytic osmylation of cis and trans 2-cyclohexene-cyclohexanol (1a) and (1b) are carried out in the presence of N-methyl morpholine-N-oxide. Under the same conditions, only the cis isomer reacted, leading to triol (3). The steric hindrance of the equatorial OH group probably prevents the trans isomer from entering into such reaction. Triol (3) was also obtained from the reduction of the d...

Polycyclic aromatic hydrocarbons (PAHs) in which five-membered rings have been interspersed among the six-membered rings generally display curved p systems that can be either fully closed (fullerenes) or partially open (bowl-shaped fullerene fragments), depending on the number of five-membered rings. Both classes of compounds belong to the larger family we call ‘GEODESIC POLYARENES.’ Herein, we...

The influence of an electric field on an isolated channel or nanopore separating two compartments filled with electrolytes produces a constant ion flux through the pore. Nucleic acids added to one compartment traverse the pore, and modulate the current in a sequence-dependent manner. While translocation is faster than detection, the α-hemolysin nanopore (α-HL) successfully senses base modificat...

Osmium tetroxide is by far the most versatile catalyst for cis-dihydroxylation (DH) of double bonds.[1, 2] When homogeneous catalysts are used, free OsO4 is always present in some step of the catalytic cycle, and the high toxicity and volatility of OsO4 have hitherto obstructed industrial application. Previous attempts to immobilize OsO4 used polymers, for example, with coordination of OsO4 on ...