The osmotic fragility of erythrocytes is known to undergo alterations when whole blood is incubated under aseptic conditions for 24 hours. The osmotic fragility of spherocytes increases considerably ( 1 ). Normal erythrocytes undergo a slight increase in fragility (2) and by contrast the osmotic fragility of leptocytes and certain other erythrocytes (3) is decreased. The sequence of events asso...

in vitro antioxidant and erythrocyte protecting activities by aqueous extract of ficus thonningii leaves on blood cells were studied in acetaminophen treated rats. the extract was safe at limit dose of 5000 mg kg-1body weight. the extract demonstrated dose dependent antihemolytic effect at dose levels between 50 and 200 mg kg-1 body weight. the lowest antihemolytic effect was observed at dose l...

Although it is recognized that red cells lose membrane during storage, estimation of the osmotic fragility of erythrocytes has not previously proven to be a useful measurement of the storage lesion. Erythrocytes from blood stored in CPD-A2 were found to have a markedly increased osmotic fragility. A major portion of this increase was found to be due to accumulation of lactate, which is only slo...

The aim of our experiments was to characterise and to validate the osmotic fragility test when applied to human blood samples with no significant alterations of osmotic fragility but with a differentiating shape of the haemolysis curve. All experiments were carried out on human erythrocytes taken from the Regional Centre of Blood Donation and Blood Therapy in Wrocław. The washed erythrocytes we...

Background: People occasionally undergo medical diagnostic X-ray examinations and expose their red blood cells to radiation. Radiation that is generated from medical diagnostic X-ray machines is widely used in medical diagnoses. One of the important parameters is kilo-voltage (kV) that is applied across the X-ray tube in medical diagnostic X-ray machines. Kilo-voltage influences the radiation d...

The addition of lead to normal human blood was previously found to cause a decrease in erythrocyte osmotic fragility in vitro. The mechanism of the decreased osmotic fragility caused by lead has not been completely clarified, but the following hypothesis has been proposed. Lead causes a leakage of water from erythrocytes, thus more water can enter the cell before haemolysis occurs. There has be...

Abnormalities in osmotic fragility have been observed in hereditary spherocytosis, hereditary elliptocytosis, pyruvate kinase deficiency, thalassaemia and iron-deficient red cells (1). Harris et al have reported a decrease in osmotic fragility of the red cells of sickle cell anaemia patients from Africa (2). The Indian patients are genetically and clinically different from the African patients ...

background: the capacity of human hbaa erythrocytes of non-malarious blood to withstand osmotic stress in the presence of five antimalarial drugs, chloroquine phosphate, quinine, fansidartm, coartemtm and halfantm was studied in vitro. materials and methods: aqueous solutions of four increasing concentrations of the drugs used in this investigation were in the order: 0.2%, 0.4%, 0.6% and 0.8% (...

Burc Molecular Genetik Tani ve Arastirma Laboratuvari Vali Konagi Cad. Orhan Ersek Sok. 19/2 34365 NisantasiŞişli, İstanbul, Turkey Tel: 902122919420, Fax: 902122472242 Aim: The aim was to investigate the interactions of hyperbaric oxygenation (HBO) and sulphur dioxide (SO2) inhalation (at 10 ppm, one hour daily for 45 days) on lipoperoxidation and fragility of RBC membranes. Methods: Malonyldi...

I T HAD BEEN ESTABLISHED by previous observations”2 that heating normal human red blood cells increases their osmotic and mechanical fragiities, and results in their sequestration in the spleen37 and liver.8 Human erythrocytes undergo budding, and the heated red cells subdivide into fragments having a decreased ratio of surface volume and increased osmotic fragility.’ Kimber and Lander8 have sh...