The challenges associated with translating health services and policy research (HSPR) evidence into practice are many and long-standing. Indeed, those challenges have themselves spawned new areas of research, including knowledge translation and implementation science. These sub-disciplines have increased our understanding of the critical success factors associated with the uptake of research ev...

Bacterial SPOR domains bind peptidoglycan (PG) and are thought to target proteins to the cell division site by binding to "denuded" glycan strands that lack stem peptides, but uncertainties remain, in part because septal-specific binding has yet to be studied in a purified system. Here we show that fusions of GFP to SPOR domains from the Escherichia coli cell-division proteins DamX, DedD, FtsN,...

This is the introductory paper in a collection of four papers on Strategy for Patient-Oriented Research (SPOR) Evidence Alliance, pan-Canadian research initiative that was funded by Canadian Institutes Health September 2017. Here, we introduce SPOR enterprise Canada, provide rationale creation information mandate and approach, describe how Alliance adds to health ecosystem Canada beyond.

Enterotoxin-producing Clostridium perfringens type A isolates are an important cause of food poisoning and non-food-borne human gastrointestinal diseases, e.g., sporadic diarrhea (SPOR) and antibiotic-associated diarrhea (AAD). The enterotoxin gene (cpe) is usually chromosomal in food poisoning isolates but plasmid-borne in AAD/SPOR isolates. Previous studies determined that type A SPOR isolate...

Escherichia coli cell division is a complex process involving over 30 proteins that are recruited to the division site. One of the important division proteins is FtsN, which has a C-terminal peptidoglycan (PG) binding region called the SPOR domain. Most SPOR domain proteins are probably involved in bacterial cell division, but their precise roles in this process are not known. Although the stru...

Of the known essential division proteins in Escherichia coli, FtsN is the last to join the septal ring organelle. FtsN is a bitopic membrane protein with a small cytoplasmic portion and a large periplasmic one. The latter is thought to form an alpha-helical juxtamembrane region, an unstructured linker, and a C-terminal, globular, murein-binding SPOR domain. We found that the essential function ...

The Strategy for Patient Oriented Research (SPOR) Evidence Alliance is a research initiative in Canada whose mission to promote the synthesis, dissemination, and integration of results into health care public decision-making clinical practice. aim this paper ( i) outline governance committee structure SPOR Alliance, ii) procedures patient system decision-maker engagement, iii) present capacity-...

BACKGROUND Diabetes is a chronic disease with increasing prevalence worldwide. Although research has improved its treatment and management, little is known about which research topics matter to people living with diabetes, particularly among under-represented groups. OBJECTIVES To explore the importance of research topics among a diverse range of people living with any type of diabetes or car...