the aim of the study was to compare the parthenogenetic activation and in vitro fertilization (ivf) of in vitro matured caprine oocytes. a total of 881 cumulus-oocyte complexes (coc’s) were collected from 243 ovaries. oocytes were matured in tcm-199 medium containing ecg (20 iu/ml), hcg (20 iuμg/ml), oestradiol-17β (1 μg/ml), bsa embryo tested (3 mg/ml) supplemented with 10% fetal bovine serum ...

background: in vitro maturation (ivm) of oocytes is a promising technique to reduce the costs and avert the side-effects of gonadotropin stimulation for in vitro fertilization (ivf). the pregnancy rates from oocytes matured in vitro are much lower than those of in vivo stimulation cycles, indicating that optimization of ivm remains a challenge. objective: in this study, we investigated the effe...

rate of in vitro maturation of oocytes is one of the challenges of assisted reproductive techniques. in this study we investigated the effects of supplementation of cysteamine on the rate of in vitro maturation of oocytes in two different media. germinal vesicle oocytes were collected from mouse ovary and cultured in two media (tcm199 and meme) with 0, 50, 100, 200, 500 µm/ml cysteamine. number...

introduction: in vitro maturation and development of immature oocytes, as an artificial reproduction technique, is useful especially in women who are affected by cancer and polycystic ovary syndrome. despite using many types of in vitro media, an appropriate environment has not been reported yet. present study was designed to assess the effect of heated human follicular fluid (hhff), which is s...

introduction: superovulation produces complications in some patients, so invitro maturation of oocytes is used to decrease or eliminate these complications and improve ivf. moreover, ivm is used for different aspects of reproductive researches. slaughterhouse ovaries are the main source of oocytes for ivm and ivf studies. different media has been introduced and experimented for in vitro maturat...

Background: in vitro maturation (IVM) of oocytes has increasing potential applications in assisted reproductive technology (ART), during which germinal vesicle (GV) oocytes cultured in vitro to produce mature (MII arrested) ones. Although functional, IVM oocytes have low developmental competence compared to in vivo matured oocytes; possibly because IVM comprises asynchrony between nuclear and c...

background: it is important to protect oocytes and embryos from oxidative stress in the culture medium. melatonin has been shown to be a direct free radical scavenger. objective: effect of melatonin during in vitro oocyte maturation, fertilization and embryo development of mouse oocytes was evaluated. materials and methods: oocytes from supper-ovulated mouse were divided to two groups: cumulus ...

The aims of the present study were to assess the effects of cysteamine as an anti-oxidant on the rate of in vitro maturation (IVM) of buffalo oocytes (experiment 1), and their viability and nuclear status following vitrification (experiment 2). Immature oocytes with compact cumulus cells obtained from the ovaries of slaughtered animals were harvested and then cultured in the maturation medium w...

background   objective:   materials and methods   results   conclusion   key words:   . vitrification, viability, in vitro maturation, gvbd, oocyte, mouse : these results suggest that step-wise vitrification of gvbd oocytes as compared to single step vitrification was better in the rate of survival and in vitro maturation of oocytes. : all non-vitrified oocytes were viable after 24 hr; however,...