Backgroud: Oocyte cryopreservation is critical in assisted reproduction techniques. However, the effect of cryopreservation on the oocyte has not been widely explored yet. Currently a thorough understanding of oocyte cryobiology is urgently required due to its far lag behind the forces propelling the clinical application of oocyte cryopreservation. Methods: In this study, we used two different ...

BACKGROUND The average age of women bearing their first child has increased strongly. This is an important reproductive health problem as fertility declines with increasing female age. Unfortunately, IVF using fresh oocytes cannot compensate for this age-related fertility decline. Oocyte freezing could be a solution. METHODS We used the Markov model to estimate the cost-effectiveness of three...

Background: The slow-freezing method is one of the most popular techniques to freeze human oocytes and it is also used in routine in vitro-fertilization (IVF) programs, particularly in those countries where embryo freezing is not allowed. Slow-freezing damages some of the structures of the egg, among which the meiotic spindle (MS) and the zona pellucida (ZP). Polarized Light Microscopy (PLM) pe...

BACKGROUND Although cryopreservation of semen is a routine procedure for preserving male gametes, an efficient method of preserving fertility through oocyte freezing has only recently become available for women. In view of the limited female reproductive lifespan, oocyte freezing can now offer women some protection against the decline in fertility with aging. METHODS A survey was performed in...

BACKGROUND A number of factors influence the success of oocyte cryopreservation and subsequent ICSI. The aim of the present study is to establish the ideal time, after oocyte retrieval, for human oocyte cryopreservation via slow freezing/rapid thawing protocol with 0.3 M sucrose concentration in cryoprotectant solution (SF/RT 0.3 M). METHODS Retrospective study with 75 patients on the clinica...

OBJECTIVE To evaluate the safety and risk of cryopreservation in female fertility preservation. DATA SOURCES The data analyzed in this review were the English articles from 1980 to 2013 from journal databases, primarily PubMed and Google scholar. The criteria used in the literature search show as following: (1) human; embryo; cryopreservation/freezing/vitrification, (2) human; oocyte/immature...

Reproductive aging has become an important social and medical development. the trend to delay childbearing in modern societies to a much less fecund age often confronts women with difficulties to conceive. the aging of the ovary resulting in a decline in the total number of oocytes and in their quality cannot be undone. However, it often engenders unrealistic expectations of what modern fertili...

Background: Vitrification of oocyte is one of the most important topics in the field of assisted reproductive techniques (ART). Considering the importance of oocyte vitrification in clinics, in the present study the effect of vitrification on mouse oocytes survivale rate and apoptosis by cryotop were investigated. Materials and Methods: 200 GV oocytes and 200 MII oocytes were obtained respectiv...