In the present study, using anti-sense oligonucleotides the inhibition of expression of the PML protein hasbeen investigated. The anti-sense oligonucleotides were designed against the translation initiation site ofthe PML gene, and their effects were investigated on cellular growth and DNA synthesis. Incubation of normalhuman fibroblast cells with the anti-sense oligonucleotid...

Oligonucleotides containing cleavable linkers have emerged as versatile tools to achieve stimulus-responsive and site-specific cleavage of DNA. However, the limitations previously reported including photolabile disulfide restricted their applications in vivo. Inspired by cathepsin B-sensitive dipeptide antibody–drug conjugates (ADCs) such Adcetris, we developed Val-Ala-02 Val-Ala-Chalcone phosp...

Oligonucleotides able to hybridize bacterial RNA via in situ hybridization may potentially act as new antimicrobials, replacing antibiotics, and fast vivo diagnostic probes, outperforming current clinical methodologies. Nonetheless, oligonucleotides are not efficiently permeate the multi-layered envelope reach their target cytosol. Cationic fusogenic liposomes here suggested vehicles enable int...

Double-stranded RNA-mediated interference (RNAi), antisense oligonucleotides (ASO), and ribozymes have excellent specificity to their target oncogenic mRNA. They also seem show great promise when it comes treating cancer. The problem is that RNAi, ASO, poor stability are constantly being degraded by nucleases. Researchers made some efforts increase oligonucleotides’ creating phospharimidate Pho...

Alteration of adipocyte function contributes to the pathogenesis metabolic diseases including Type 2 diabetes and insulin resistance. This highlights need better understand molecular mechanism involved in dysfunction develop new therapies against obesity-related diseases. Modulating expression proteins micro-RNAs adipocytes remains highly challenging. paper describes a protocol differentiate mu...

background: dendritic cells (dcs) are ideal accessory cells in the field of gene therapy. delivery of dna and sirna into mammalian cells is a useful technique in treating various diseases caused by single gene defects. selective gene silencing by small interfering rnas (sirnas) and antisense oligodeoxynucleotides (odn)s is an efficient method for the manipulation of cellular functions. an effic...

background: malignant melanoma is a highly metastatic cutaneous cancer and typically refractory to chemotherapy. deregulated apoptosis has been identified as a major cause of cancer drug resistance, and upregulated expression of the inhibitor of apoptosis protein melanom, an inhibitor of apoptosis (ml-iap) is frequent in melanoma. methods: based on the conclusion that ml-iap expression contribu...

The first oligonucleotide therapeutic was approved by the Food and Drug Administration in 1998, since then, 12 nucleic acids have been commercialised as medicines. To be approved, oligonucleotides need to identified characterised well its related impurities. Different methods exist, but most commonly used is ion-pairing reversed-phase liquid chromatography with tandem mass spectrometry. separat...

Background: Malignant melanoma is a highly metastatic cutaneous cancer and typically refractory to chemotherapy. Deregulated apoptosis has been identified as a major cause of cancer drug resistance, and upregulated expression of the inhibitor of apoptosis protein melanom, an inhibitor of apoptosis (ML-IAP) is frequent in melanoma. Methods: Based on the conclusion that ML-IAP expression contribu...