Carbapenem-hydrolyzing oxacillinases are reported increasingly in Acinetobacter baumannii. Since they hydrolyze carbapenems at low levels, the roles of carbapenem-hydrolyzing oxacillinases OXA-23, OXA-40, and OXA-58 in A. baumannii were determined. The bla(OXA-23), bla(OXA-40), and bla(OXA-58) genes were inserted in broad-host-range plasmid pAT801 and transformed in Escherichia coli DH10B and i...

Acinetobacter baumannii is one of the major threats in clinical infections due to its antibiotic resistance ability. It shows increasing resistance to carbapenems, mainly due to β-lactamase mediated mechanisms. The aim of this study was to investigate carbapenem resistance (CR) profiles and analyze β-lactamases genes composition of clinical A. baumannii strains from a teaching hospital in Xi'an...

The purpose of this study was to identify the genes coding for resistance to ceftazidime and imipenem and describe the molecular epidemiology of A. baumannii strains isolated from a clinical center in Colombia. Twenty isolates of imipenem-resistant A. baumannii from an equal number of patients with nosocomial infections were obtained. Primers were used to amplify genes bla IMP, bla VIM, bla OXA...

Our study determines the resistance gene profile of a set of Acinetobacter baumannii hospital isolates. A. baumannii is responsible for nosocomial outbreaks and sporadic infections. We extracted and PCR amplified bacterial DNA isolated from patients with ages below 60 years (23.36%) and above 60 years (76.64%). Most of the patients were admitted in the ICU (36.13%) and pneumology departments (2...

Acinetobacter baumannii is an important nosocomial pathogen occurring particularly in intensive care (ICU) as well as burn therapy units (BTU). A. baumannii strains have emerged as resistant to almost all antimicrobial agents, including carbapenems. b-lactamase-mediated resistance is the most common mechanism for carbapenem resistance in this species. Carbapenem-hydrolysing class D b-lactamases...

Carbapenem-resistant Acinetobacter baumannii (CR-AB) causes serious nosocomial infections, especially in ICU wards of hospitals, worldwide. Expression of bla OXA genes is the chief mechanism of conferring carbapenem resistance among CR-AB. Although some bla OXA genes have been studied among CR-AB isolates from Iran, their bla OXA-23-like genes have not been investigated. We used a multiplex-PCR...

Technical Appendix Table. β-lactamase profiles of multidrug-resistant Gram-negative bacilli isolated in 2010 and 2011, France* Region of origin No. culture-positive patients/total no. patients Species No. isolates β-lactamase ESBL Carbapenemase pCASE Africa 33/87 E. coli 18 CTX-M-15 2 CTX-M-1 2 SHV-12 1 CTX-M-3 OXA-48 1 CTX-M-14 1 CTX-M-15 TEM-169 1 CTX-M-15 CMY-2 1 CTX-M-24 OXA-48 1 ND K. pneu...

A collection of 15 carbapenem-resistance Acinetobacter baumannii clinical isolates was analysed on two tertiary hospitals in Mexico. The OXA-51 was identified in all isolates, followed by OXA-239 and OXA-58; OXA-239 is described as a new OXA-23-like allele. These carbapenemases were identified on four clonal groups, distributed between two neighbouring hospitals. Acinetobacter baumannii is poor...

conclusions the present study showed that bla oxa-23-like was the most frequent carbapenemase identified among carbapenem-resistant a. baumannii isolated in tehran hospitals. evaluation of antibiotic resistance genes in a. baumannii, is necessary to control further dissemination of these antibiotic resistant genes. results results showed that 123 of 131 (93.89%) acinetobacter species, possessed...

We investigated the resistance determinant of 13 clinical isolates of carbapenem-resistant Acinetobacter baumannii collected from a regional hospital in the north of Thailand. All isolates were multidrug resistant and produced the OXA-23 carbapenemase. The bla(OXA-23) gene was found adjacent to ISAba1. Furthermore, two isolates carried the metallo beta-lactamase gene, bla(IMP). The bla(OXA-23) ...