The Origin Recognition Complex (ORC) is thought to be required for the initiation of DNA replication in all eukaryotes. In Saccharomyces cerevisiae, ORC is bound to origins of DNA replication throughout the cell cycle and directs the assembly of higher-order protein-DNA complexes during G1. I have investigated the architecture of yeast ORC bound to origin DNA. Determination of DNA residues impo...

The origin recognition complex (ORC) marks chromosomal sites as replication origins and is essential for replication initiation. In yeast, ORC also binds to DNA elements called silencers, where its primary function is to recruit silent information regulator (SIR) proteins to establish transcriptional silencing. Indeed, silencers function poorly as chromosomal origins. Several genetic, molecular...

The origin recognition complex (ORC) is conserved in all eukaryotes. The six proteins of the Saccharomyces cerevisiae ORC that form a stable complex bind to origins of DNA replication and recruit prereplicative complex (pre-RC) proteins, one of which is Cdc6. To further understand the function of ORC we recently determined by single-particle reconstruction of electron micrographs a low-resoluti...

The origin recognition complex (ORC) is an essential DNA replication initiation factor conserved in all eukaryotes. In Saccharomyces cerevisiae, ORC binds to specific DNA elements; however, in higher eukaryotes, ORC exhibits little sequence specificity in vitro or in vivo. We investigated the genome-wide distribution of ORC in Drosophila and found that ORC localizes to specific chromosomal loca...

The origin recognition complex (ORC) binds sites from which DNA replication is initiated. We address ORC binding selectivity in vivo by mapping ∼52,000 ORC2 binding sites throughout the human genome. The ORC binding profile is broader than those of sequence-specific transcription factors, suggesting that ORC is not bound or recruited to specific DNA sequences. Instead, ORC binds nonspecifically...

Recently, a promising programming model called Orc has been proposed to support a structured way of orchestrating distributed web services. Orc is intuitive because it offers concise constructors to manage concurrent communication, time-outs, priorities, failure of sites or communication and so forth. The semantics of Orc is also precisely defined. However, there is no verification tool availab...

DNA replication, as with all macromolecular synthesis steps, is controlled in part at the level of initiation. Although the origin recognition complex (ORC) binds to origins ofDNAreplication, it does not solely determine their location. To initiate DNA replication ORC requires Cdc6 to target initiation to specific DNA sequences in chromosomes andwithCdt1 loads the ring-shaped mini-chromosome ma...

BACKGROUND This study aimed to investigate the relationship between oxidized regenerated cellulose (ORC) and mucosa healing in an experimental animal model. METHODS Fifteen adult Sprague-Dawley rats were randomly divided into three groups that underwent different wound treatments. In Group 1, no pharyngeal wound was created. In Group 2, the pharyngeal wound was sutured with Prolene only. In G...

For Orc, a language for orchestrating concurrent computations, a “token semantics” is presented. This semantic model preserves the original program text during evaluation, rather than reducing the given program to its evaluated value.