For two libraries (PDF1 and ODF1) using Illumina sequencing 44,082,301 and 43,708,132 clean reads were obtained, respectively. After being mapped to the bovine RefSeq database, 15,533 genes were identified to be expressed in both types of follicles (cut-off RPKM > 0.5), of which 719 were highly expressed in bovine follicles (cut-off RPKM > 100). Furthermore, 83 genes were identified as being di...

Obstruction of the male reproductive tract commonly results in generation of antisperm autoantibodies. However, only a few of the sperm autoantigens recognized by these antibodies have been characterized. To identify postobstruction rat sperm autoantigens, sperm proteins were separated by two-dimensional(2-D) gel electrophoresis. Spots corresponding to proteins that were stained by at least 50%...

We have isolated the human homolog of the rat Odf2 gene. In rat, Odf2, the 84-kDa major outer dense fiber protein, interacts strongly and specifically with Odf1, the 27-kDa major outer dense fiber protein. The interaction is mediated by leucine zippers during ODF assembly along the sperm axoneme. We compared homology and genomic structure to rat and mouse Odf2 genes. Using fluorescence in situ ...

Background According to a worldwide survey in 2010 infertility affects 48.5 million of couples. In roughly half of the cases infertility is provoked by the male mate. Thus, a significant percentage of young men are infertile but the underlying causes are mostly unknown. Male fertility and reproduction success critically depends on proper formation of the mature sperm. Transmission of the geneti...

IgA nephropathy, also known as Berger's disease, is the most common primary glomerulonephritis and a major cause of end stage renal disease (ESRD). IgA nephropathy typically presents as macroscopic haematuria (blood in the urine), often following a mucosal infection, and diagnosis is made based on a renal biopsy which shows deposition of IgA-containing immune complexes in the mesangial area of ...

The effects of RPF on metabolites and reproductive genes in testes Malin ram was investigated. Twenty rams (36.6kg ± 5.57 kg bodyweight), were subjected to four dietary treatments; A: basal diet without rumen-protected fat (RPF), B; with 2% prilled fat, C; calcium salt D; canola oil. At the end experiment out five animals from each group slaughtered. excised for gene expression stud-ies. tested...

During mammalian spermatogenesis, the diploid spermatogonia mature into haploid spermatozoa through a highly controlled process of mitosis, meiosis and post-meiotic morphological remodeling (spermiogenesis). Despite important progress made in this area, the molecular mechanisms underpinning this transformation are poorly understood. Our analysis of the expression and function of the putative se...