The influence of explant nodal positions on the in vitro shoot growth and proliferation were studied in the two rose cvs. ‘Bianca’ and ‘El Torro’. Third, fourth and fifth nodal explants were cultured on the modified MS medium supplemented with 1.0 and 5.0 mg/l BA. In both the cultivars, higher rate of proliferation (‘Bianca’ 3.75; ‘El Torro’ 2.65) were obtained from the explants distal to the a...

the influence of explant nodal positions on the in vitro shoot growth and proliferation were studied in the two rose cvs. ‘bianca’ and ‘el torro’. third, fourth and fifth nodal explants were cultured on the modified ms medium supplemented with 1.0 and 5.0 mg/l ba. in both the cultivars, higher rate of proliferation (‘bianca’ 3.75; ‘el torro’ 2.65) were obtained from the explants distal to the a...

lemon balm (melissa officinalis l.) is an herbaceous perennial plant of the lamiaceae family that has a wide edible and medicinal uses. in this research the potential of five different explants (nodal segments, shoot tip, cotyledon, hypocotyl and leaf segments) on ms mediums supplemented with different concentrations of 6-benzylaminopurine (bap) (2.2, 4.4 and 8.8 µm) alone or in combination wit...

alstroemeria (alstroemeriaceae family) is an ornamental monocot mainly produced for its cut flowers. embryogenic calluses have been employed for gene transformation in alstroemeria. this research work was undertaken to study the embryogenic callus induction in alstroemeria cv. fuego. the vegetative explants (node, internode, leaf) along with various concentrations of auxins (picloram, naa, iaa,...

In vitro propagation of Dendrobium macrostachyum, a threatened and endemic species was achieved through nodal explants. The nodal explants were cultured on Murashige and Skoog (MS) basal medium and MS medium supplemented with N6-benzyladenine (BA-2.22, 4.44 and 8.88 microM), Kinetin (KN-2.32, 4.65, and 9.29 microM) and Coconut water (CW, 5, 10 and 15%) individually or in combination with 2.69 m...

In vitro multiple shoot regeneration of Solanum nigrum L., an Indian medicinal plant was accomplished on MS medium utilizing shoot tip and nodal explants. Direct multiple shoots differentiated within 6 weeks when explants were cultured on MS medium containing BAP (1.0-5.0 mg/l) and KIN (1.0-5.0 mg/l) individually. Among various concentrations of cytokinins tested, maximum number of multiple sho...

in vitro multiple shoot regeneration of solanum nigrum l., an indian medicinal plant was accomplished on ms medium utilizing shoot tip and nodal explants. direct multiple shoots differentiated within 6 weeks when explants were cultured on ms medium containing bap (1.0-5.0 mg/l) and kin (1.0-5.0 mg/l) individually. among various concentrations of cytokinins tested, maximum number of multiple sho...

dog rose (rosa canina) has been using as a rootstock for ornamental roses also it is one of the medicinal plants. it can be propagated under in vitro conditions. after removing chilling requirement of buds, axillary wood buds of dog rose were cut in three nodal positions (lower, middle and terminal) on the stem and then explants after decontamination, cultured on murashige and skoog medium (ms ...

the present investigation was conducted to study the effects of type and concentration of auxin and cytokinin on callus induction of three strawberry (kurdistan, parose and camarosa) cultivars. for this purpose, leaf blade, nodal, petiole, receptacle, stamen and flower bud explants were cultured on murashige and skoog (ms) medium at three separate experiments included 2, 4 dichlorophenoxyacetic...