the influence of explant nodal positions on the in vitro shoot growth and proliferation were studied in the two rose cvs. ‘bianca’ and ‘el torro’. third, fourth and fifth nodal explants were cultured on the modified ms medium supplemented with 1.0 and 5.0 mg/l ba. in both the cultivars, higher rate of proliferation (‘bianca’ 3.75; ‘el torro’ 2.65) were obtained from the explants distal to the a...

The influence of explant nodal positions on the in vitro shoot growth and proliferation were studied in the two rose cvs. ‘Bianca’ and ‘El Torro’. Third, fourth and fifth nodal explants were cultured on the modified MS medium supplemented with 1.0 and 5.0 mg/l BA. In both the cultivars, higher rate of proliferation (‘Bianca’ 3.75; ‘El Torro’ 2.65) were obtained from the explants distal to the a...

This study establishes a protocol for the induction of somatic embryogenesis in Anthurium andraeanum cv. Eidibel. The experiment was arranged in a completely randomized 5 x 5 x 5 factorial design using five explant types (whole leaves, half leaves; petiole; nodal segments and root segments) from in vitro plantlets; five auxins: indole-3-acetic acid (IAA), α-naphthaleneacetic acid (NAA), indole-...

the present investigation was conducted to study the effects of type and concentration of auxin and cytokinin on callus induction of three strawberry (kurdistan, parose and camarosa) cultivars. for this purpose, leaf blade, nodal, petiole, receptacle, stamen and flower bud explants were cultured on murashige and skoog (ms) medium at three separate experiments included 2, 4 dichlorophenoxyacetic...

the present work developed an efficient plant regeneration and propagation system via direct organogenesis for morinda officinalis how., a valuable endangered medicinal plant. the effects of explant types on shoot induction, plant growth regulators on shoot proliferationand elongation and the subsequent rooting ability of shoots were examined. among the various types of explant, direct shoot pr...

Shoot multiplication of Bupleurum distichophyllum was achieved from the nodal and shoot tip explants of mature plants using MS with different concentrations and combinations of growth regulators. Maximum explant response was from axillary shoots and the highest number of shoots per explant was obtained on MS fortified with 1.0 mg/l BAP. The highest degree of axillary shoot proliferation was fou...

Surface sterilization is a vital step in preparation of healthy and viable explants tissue culture. Most surface contaminants can be eliminated by with suitable sterilizing agent. The study aimed to present an effective disinfection method for Clinacanthus nutans shoot regeneration using nodal segments. A total four different approaches were conducted treating various concentrations Sterilizing...

In vitro propagation of Dendrobium macrostachyum, a threatened and endemic species was achieved through nodal explants. The nodal explants were cultured on Murashige and Skoog (MS) basal medium and MS medium supplemented with N6-benzyladenine (BA-2.22, 4.44 and 8.88 microM), Kinetin (KN-2.32, 4.65, and 9.29 microM) and Coconut water (CW, 5, 10 and 15%) individually or in combination with 2.69 m...