Nociceptin/orphanin FQ and nocistatin are two neuropeptides with opposing effects on spinal neurotransmission and nociception. Nociceptin/orphanin FQ selectively suppresses excitatory glutamatergic neurotransmission, while nocistatin selectively interferes with glycinergic and gamma-aminobutyric acid (GABA)-ergic transmission. Here, we performed whole-cell patch-clamp recordings from superficia...

Received December 30, 2001 A neuropeptide nociceptin or orphanin FQ (Noc/OFQ) was previously identified as an endogenous ligand for the orphan opioid receptor-like receptor. Studies on the analysis of the precursor of Noc/OFQ, we identified another novel neuropeptide and named it “nocistatin”. Noc/OFQ is involved in a broad range of pharmacological actions in various tissues from the central ne...

Nociceptin/orphanin FQ (N/OFQ), nocistatin, and prepro-N/OFQ 160-187 (C-peptide) are all derived from the same precursor protein. We examine the pharmacological mechanisms of nocistatin- and C-peptide-induced pronociceptive responses in a novel algogenic-induced nociceptive flexion test in mice. The intraplantar (i.pl.) injection of nocistatin- and C-peptide induced pronociceptive responses in ...

From Department of Medical Chemistry, Kansai Medical University, Moriguchi, Japan; Department of Biomedical Engineering, Osaka Institute of Technology, Osaka, Japan; Department of Anesthesiology, Osaka Medical College, Takatsuki, Japan; Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan; Laboratory for Animal Resources and Genetic Engineering, RIKEN ...

Tissue or nerve injury can dramatically alter the transmission of sensory stimuli by spinal cord neurons, so that a light touch produces pain. The discovery that peptide products of prepronociceptin processing either facilitate or inhibit these mechanisms suggests novel approaches to treating these conditions.

I. Deák Beáta, Klukovits Anna, Kormányos Zsolt, Falkay György, Gáspár Róbert. A nociceptin és nocisztatin hatása a terhes humán és patkány uterusz kontraktilitására. A Magyar Élettani Társaság (MÉT) és a Magyar Kísérletes és Klinikai Farmakológiai Társaság (MFT) II. Közös tudományos konferenciája; Szeged, 2010. június 16-18. II. Beáta H Deák, Anna Klukovits, Eszter Ducza, Zsolt Kormányos, Attil...

a new biological active hexapeptide of c-terminal of nocistatin, contains glu-gln-lys-gln-leu-gln sequence was synthesized according to solid phase peptide synthesis on the surface of 2-chloro tritylchloride resin and using fmoc-protected amino acids in the presence of tbtu (o-(benzotriazol-1-yl)-n,n,n',n'-tetramethyl uranium tetrafluoroborate) as a coupling reagent. then, amidation o...

The role of nociceptinergic transmission in the nucleus tractus solitarii (NTS) in the central modulation of cardiovascular activity was investigated in pentobarbital-anesthetized and conscious rats. Pharmacological activation of nociceptin receptors with a unilateral injection of synthetic nociceptin into the NTS, wherein injection of L-glutamate (1 nmol) caused typical depressor responses, el...