chronic granulomatous disease (cgd) is a rare inherited disorder characterized by inability of phagocytes to generate oxygen radicals needed for intracellular killing of phagocytic microorganisms. we report a 2.5-year-old iranian female with multiple liver abscesses. she was admitted in surgical ward because of abdominal pain and fever for one month duration that had no response to conventional...

L-lactate, a key metabolite of the anaerobic glycolytic pathway, plays an important role as a biomarker in medicine, in the nutritional sector and food quality control. For these reasons, there is a need for very specific, sensitive, and simple analytical methods for the accurate L-lactate measuring. A new highly selective enzymatic method for L-lactate determination based on the use of flavocy...

In this new method for determining serum guanase activity by use of the Hitachi 736-40 automated analyzer, serum is incubated with a mixture of xanthine oxidase, superoxide dismutase, and catalase; a reagent containing KCN, guanine, nitrotetrazolium blue, and Triton X-100 is added; and the increase in absorbance at 570 and 660 nm is measured for 2.4 min. Only 20 microL of sample is required, an...

In this simple and direct method for determining total bile acids in serum, the serum was mixed with sodium pyruvate, a lactate dehydrogenase blocker, and bile acids were then measured spectrophotometrically after the following enzyme reaction. Bile acids are converted to 3-oxo bile acids with 3 alpha-hydroxysteroid dehydrogenase (EC 1.1.1.50) with concomitant reduction of NAD+ to NADH. The hyd...

Industrial pollutants induce the production of toxic reactive oxygen species (ROS) such as O2.-, H2O2, and ·OH in plants, but they have not been well quantified or localized in tissues and cells. This study evaluated the pollutant- (HSO3-, NH4NO3, Al3+, Zn2+, and Fe2+) induced toxic effects of ROS on the aerial roots of Chinese banyan (Ficus microcarpa). Root cell viability was greatly reduced ...

and Fabry's diseases, as well as metachromatic leucodystrophy). Leucocyte studies of families have done much to increase our knowledge of the genetics of the inherited metabolic disorders and in many cases the heterozygous state can be clearly delineated using these readily available cells. Direct enzyme assay on the isolated cells is usually satisfactory, although in some cases the white cell ...

A histo-enzymatic technique for visualizing and quantifying endogenous NAD(H) in brain tissue was developed, based on coupled enzymatic cycling reactions that reduce nitrotetrazolium blue chloride to produce formazan. Conditions were used where the endogenous level of nicotinamide adenine dinucleotides (NAD(H)) was the rate limiting factor for formazan production. Spontaneous degradation of NAD...

Fabrication of the heterojunction photocatalyst with appropriate band potentials as a promising method inhibiting electron-hole pair recombination leading to enhanced photocatalytic properties. Herein, BiOBr, Bi 4 O 5 Br 2 , and binary BiOBr/Bi composite were selectively synthesized by employing one-step microwave irradiation method. Then, double Z-scheme FeVO /Bi /BiOBr ternary composites diff...

  Chronic granulomatous disease (CGD) is a rare inherited disorder characterized by inability of phagocytes to generate oxygen radicals needed for intracellular killing of phagocytic microorganisms. We report a 2.5-year-old Iranian female with multiple liver abscesses. She was admitted in surgical ward because of abdominal pain and fever for one month duration that had no response to conventio...