Detailed analysis of the expression pattern of seven legumain genes from Nicotiana tabacum L. cv. SNN revealed that it showed neither coincidences with the branches of the phylogenetic tree of legumains nor with their conventional assignment to organs. This agreed well with the fact that, so far, no functional differences could be assigned to the sequence differences reflected in the branches o...

Phosphorylation of Tobacco mosaic virus movement protein (TMV-MP) at three carboxyterminal Ser/Thr sites negatively regulates TMV-MP gating function and viral spread in Nicotiana tabacum but not in Nicotiana benthamiana, indicating a host dependant inactivation strategy. Here, we examine the effect of mimicking carboxyterminal phosphorylation on cell-to-cell transport of TMV-MP protein itself i...

molecular farming has been considered as a secure and economical approach for production of biopharmaceuticals. human tnf related apoptosis inducing ligand (trail) as a promising biopharmaceutical candidate has been produced in different expression hosts. however, little attention has been paid to molecular farming of the trail in spite of numerous advantages of plant expression systems. theref...

We report cell-to-cell movement of mitochondria through a graft junction. Mitochondrial movement was discovered in an experiment designed to select for chloroplast transfer from Nicotiana sylvestris into Nicotiana tabacum cells. The alloplasmic N. tabacum line we used carries Nicotiana undulata cytoplasmic genomes, and its flowers are male sterile due to the foreign mitochondrial genome. Thus, ...

We used next generation sequencing to characterize and compare the genomes of the recently derived allotetraploid, Nicotiana tabacum (<200,000 years old), with its diploid progenitors, Nicotiana sylvestris (maternal, S-genome donor), and Nicotiana tomentosiformis (paternal, T-genome donor). Analysis of 14,634 repetitive DNA sequences in the genomes of the progenitor species and N. tabacum revea...

The CLE family (CLAVATA3/embryo surrounding region-related), a class of small secreted proteins, play important roles in plant development and stress responses. Members the have been characterized number species, including Arabidopsis rice. However, limited information is available about peptides tobacco (Nicotiana tabacum) related Nicotiana species. Here we report identification 84 members thr...

ClC Cl(-) channels are found in almost all organisms, ranging from bacteria to mammals, in which nine Cl(-) channels belonging to the ClC family have been identified. The biophysical properties and physiological functions of ClC Cl(-) channels have been extensively reviewed. In this short review, we will focus on recent results obtained on the X-ray structure and functional properties of the pr...

effects of excess concentrations of boron on major cell wall components of tobacco cells(nicotiana tabacum l. cv. burley 21) were studied. pectin, xyloglucan, hydroxyproline-richglycoproteins (extensin), and arabinogalactan proteins were characterized. results showedthat increased boron supply resulted in significant decrease in cell and cell wall dry weights.also, high concentrations of boron ...

Epitrix hirtipennis (Melsheimer, 1847) (Coleoptera: Chrysomelidae: Galerucinae: Alticini), a leaf beetle native to North America, is recorded for the first time from Hungary. Several specimens were found on tobacco (Nicotiana tabacum L.) plantations in eastern and central parts of country. Its life cycle importance as pest are briefly discussed.