background: the immunomagnetic separation technique is the basis of monocyte isolation and further generation of monocyte-derived dendritic cells. objective: to compare the efficiency of monocyte positive and negative separation, concentration of beads, and their impact on generated dendritic cells. methods: monocytes were obtained using monoclonal antibody-coated magnetic beads followed the fi...

in the software development process, testing is one of the most human intensive steps. many researchers try to automate test case generation to reduce the manual labor of this step. negative selection is a famous algorithm in the field of artificial immune system (ais) and many different applications has been developed using its idea. in this paper we have designed a new algorithm based on nega...

Abstract Central tolerance aims to limit the production of T lymphocytes bearing TCR with high affinity for self-peptide presented by MHC molecules. The accumulation thymocytes such receptors is limited negative selection or diversion into alternative differentiation, including regulatory cell commitment. A role orphan nuclear receptor NR4A3 in has been suggested, but its function this process ...