An assay technique, homogeneous enzyme immunoassay, is described for the quantitative determination of morphine derivatives in biological fluids. An enzyme is labeled with morphine. When the enzymelabeled morphine is bound by antimorphine antibodies, the enzyme is rendered inactive. Free morphine competes with enzyme-morphine for antibody binding sites preventing inhibition of enzyme activity. ...

Endogenous codeine and morphine were identified in rat brain by immunological determination following HPLC. To demonstrate occurrence of a biosynthetic pathway to morphine in mammals similar to that used by the poppy plant, (+)-salutaridine, (-)-thebaine, and (-)-codeine were administered to rats intravenously. These compounds, which are intermediates in the synthesis of morphine in Papaver som...

BACKGROUND The measurement of morphine in biological samples has become a routine in many clinical and forensic toxicology laboratories. A high-performance liquid chromatography (HPLC) method was developed for the determination of morphine in plasma. METHODS Samples were extracted using Zeolite Y column followed by reversed phase HPLC with fluorescence detection. This method was based on an e...

A highly sensitive and specific method is described for quantitative determination of morphine in urine. Used in the method is a gas chromatograph and a computerized, quadrupole mass spectrometer operated under chemical ionization conditions. Morphine concentrations as low as 5 ng/mI can be measured by selected ion monitoring, with N-trideuteromethyl morphine as the internal standard. The sensi...

The estimation of morphine in biological material is difficult and tedious. Many methods have been advocated, not one of which has received the approval of successful employment by a considerable number of investigators. In the preliminary stages of projected work upon the behavior of morphine in the metabolism, it was necessary to subject existing methods to critical study for the purpose of i...

A new, sensitive, simple, inexpensive and fast kinetic spectrophotometric method was developed for the determination of trace amounts of morphine over the range of 12-60ng/mL. The method is based on the catalytic effect of morphine on the reaction of bromate and methylene bluein acidic media is reported. The reaction was monitored spectrophotometrically by 60 ng/ml measuring the decrease in abs...

Morphine is an important drug because of its usefulness in the management of pain as well as its toxicological property of producing addiction. The drug is also important as an experimental tool for defining physiological and pathological mechanisms of pain. Numerous investigators have therefore at tempted to develop procedures for the determination of morphine (1). Radioimmunoassay has been in...