by immunizing mice with killed whole bacterial cells of brucella abortus s (99), a panel of six hybridomas producing monoclonal antibodies (mab) specific for the surface antigens of this bacterium were produced. elisa was used to screen the hybridoma supernatants. immunoblots of the cell extract indicated that three mab were specific for s-lps (ba-1, ba-2, ba-3) and three others were reactive w...

despite the major advances in conventional forms of treatment (i.e. surgical techniques, radiotherapy and chemotherapy) and improved survival rates, cancer is still the second leading cause of death in developing countries. one major limitation of cytotoxic drugs and radiation in the treatment of cancer patients is their inability to discriminate between malignant and normal tissues. this in tu...

background : production of monoclonal antibodies to leishmania antigens assists the identification and characterization of these organisms. objective: production of monoclonal antibodies against epitopes on the gp63. methods: two murine monoclonal antibodies to gp63 were produced and characterized. the reactions of both antibodies with soluble leishmanial antigens, purified gp63 and truncated r...

the b-cell cd20 antigen is one of the most reliable surface targets in immunotherapy of b lymphoma. in this project, we studied the production and characterization of a new monoclonal antibody against chimeric human cd20 extra loops (hcd20 exl). the results showed that clone c12h, igg2/k isotype reacted with the antigen in elisa and immunoblot. the kd value was  found to be 2×10-9m and flow cyt...

Despite the various therapies available, the use of monoclonal antibodies is a highly specific approach that has only recently been of interest to researchers. The properties of antibodies have led to their use in the treatment of various diseases, including cancer, Alzheimer's disease, diabetes and multiple sclerosis (MS). MS, a chronic inflammatory disease, occurs commonly in young adults. Th...

background: monoclonal antibodies have been employed extensively for the identification of leishmania species, development of diagnostic tests and in the characterization of defined leishmanial antigens. objectives: identification and characterization of  leishmania spp. directly from cutaneous lesions of infected individuals. methods: an immunoperoxidase test (avidin-biotin technique) using mo...

alpha-fetoprotein (afp), a serum glycoprotein belonging to the onco-developmental proteins group, serves as a marker both in cancer research and in studies concerning fetal development and fetal pathophysiology. monoclonal anti-afp antibodies are essential reagents in developing appropriate techniques for measurement of this protein. in this study, in order to produce anti-afp monoclonal antibo...

both zoonotic and anthroponotic cutaneous leishmaniasis (cl) caused by l. major and l. tropica, respectively, are endemic in different parts of iran. this study was performed to investigate the new changes in epidemiological pattern of cl, and to identify the species of leishmania and the strains of l. major isolated from different endemic areas of iran. seventy-two isolates from 245 samples co...

background: different methods have been used for characterization of leishmania promastigotes. monoclonal antibodies are useful in characterization of   leishmania spp . both amastogotes and promastigotes. objective: comparing the characterization of   leishmania spp. promastigotes with immunoperoxidase test (avidin-biotin) techniques and an indirect immunofluorescent assay (ifa).   methods: ap...