background: dna extraction is one the most important steps for molecular analysis of food-borne pathogens. in this research, three methods of dna extractions from beef harboring sarcocystis spp. were compared for the quality, quantity, safety, as well as cost-effectiveness. methods: about 100 mg intersostal and diaphragm were collected from 10 slaughtered cattle. after ensuring their contaminat...

currently, different highly sensitive nucleic acid detection-based protocols used in medical diagnostic laboratories need specific working conditions, and if no optimization is performed, misleading results can be obtained. limitations of each protocol is different in obtaining an appropriate result. awareness of these limitations plays an important role in providing a reliable result for physi...

introduction: the main agents of sarcocystosis in cattle as an intermediate host include s. cruzi, s. hominis and s. hirsuta. a sensitive and specific tool such as molecular-based techniques would be necessary to identify the species. case report: after collection of beef sample from yazd slaughterhouse, dna extraction was done with salting out method. the 18srrna gene as a specific target gene...

background: sarcocystosis is one of the most widespread parasitic diseases in cattle caused by three main species of sarcocystis genus. the major aim of this study was to determine the prevalence of sarcocystis spp. in meat produced in yazd, central iran with special reference to species identification, using polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp).  method...

conclusions despite rapidity, automation, accuracy, cost-effectiveness, high sensitivity, and high specificity of molecular techniques, each type of molecular technology has its own advantages and disadvantages. results various types of molecular biology tools can be used to detect and quantify viral genomic elements and analyze the sequences. these molecular assays are proper technologies for ...

personalized medicine is significantly changing the fundamentals of healthcare. genetic diagnostic tests are used in various parts of medicine – from initial screening to the diagnosis and finally treatment. investigations in fields of drug development indicate that oncology may lead other therapeutic areas in the number of targeted therapies on the marketplace as well as in the pipeline, expec...

methods allplex gi-virus assay was developed to detect pathogens causing viral gastroenteritis, one of the major diseases caused by rna viruses. this one-step multiplex real-time polymerase chain reaction (pcr) based on the mudt technique permits simultaneous amplification and detection of target nucleic acids of norovirus gi, norovirus gii, rotavirus a, adenovirus f, astrovirus, and sapovirus ...