b a ckground: diagnostic study of vector ticks for different pathogens transmitted specifically have been done by iranian old scientists working on the basis of biological transmission of pathogens. in this study we decided to con- firm natural infection of different collected ticks from three different provinces of iran. m e t hods: ticks were collected from livestock (sheep, goats and cattle)...

background and objectives: contamination of cell lines and biological products is one of the major problems of cell culture techniques. rapid detection of mycoplasma contamination in cell culture is an important part of quality control standards in related laboratories. the aim of this study was to evaluate the efficacy of pcr in detection of myroplasma as contaminants in cell cultures and othe...

conclusions our findings implied that a wide range of bacteria could be involved in creating urinary tract infection in patients referred to a medical laboratory of kashani and hajar hospital in shahrekord, iran. regardless of age, sex and the use of catheter, a wide range of bacteria could be involved in urinary tract infections. patients and methods in this cross-sectional study 147 urine sam...

conclusions: the results showed that direct fusion of the recombinant plasmids containing the initial and ending parts of the target gene are simple and cost-effective techniques for increasing the length of the fragment and constructing ipc. results: the size of the pcr product was equal to the sum of the first and second fragments. sequencing confirmed the presence of the desire insert (ipc s...

fusarium oxysporum f. sp. lycopersici (fol) is the causal agent of vascular wilt in tomato, an important plant disease in iran. four monogenic resistance genes in tomato are used for identification of races of fol and their corresponding avirulence genes avr1, avr2 and avr3 were identified in pathogen one of which, avr2, is f.sp. specific. hence they can serve as reliable markers for racial ide...

The aim of this study was to isolate Clostridium difficile from dogs’ faeces, and to study the frequency of its virulence genes. A total of 151 samples of dogs’ faeces were collected. The isolation of C. difficile was performed by using the bacterial culture methods followed by DNA extraction using boiling method. Multiplex PCR method was performed for identification of tcdA, tcdB, cdtA and cdt...

Background: Congenital factor XIII (FXIII) deficiency is one of the rarest bleeding disorders with a prevalence of one per 2 million in the general population. The disorder is accompanied by a high rate of life-threatening bleeding. Due to normal results of routine coagulation tests, diagnosis of the disorder is challenging, but molecular methods can be used for precise diagnosis. Direct mutati...

  Leptospirosis is an acute infectious, systemic and septisemic disease which had recent outbreaks in some parts of Iran especially in north provinces. Rapid detection is a critical step for treatment and control of this disease. In this research a PCR based method was evaluated for detection of Iranian local endemic serovars. All reported endemic serovars of Leptospira including Leptospira gri...