the purpose of this study was to investigate the effect of additives, poly(ethylene glycol) (peg) 1450, poloxamer 407, polyvinyl alcohol (pva) and sodium chloride in order to improve physico-chemical characteristics, encapsulation efficiency and in vitro release of bovine serum albumin, form poly(d,l-lactic-co-glycolic acid) (plga) microparticles prepared by the w/o/w solvent evaporation method...

www.BioTechniques.com 159 Vol. 52 | No. 3 | 2012 Quantitative measures of physiological traits such as enzyme activity are often expressed as units of activity per milligram protein. Although numerous assays have been developed to measure protein content, including the colorimetric assays of Amido Black (1), Biuret (2), Bicinchoninic Acid (3) and Coomassie Blue (4,5), the Lowry assay (6) or its...

BACKGROUND Health care workers and individuals with frequent contact with latex are at risk for latex protein allergy. OBJECTIVE The purpose of this study was to compare several established methods for measuring protein in extracts from latex-containing medical devices. METHODS Extracts from latex gloves were analyzed for natural rubber proteins using a modified Lowry assay and two differen...

Genetically induced outer membrane particles from Gram-negative bacteria, called Generalized Modules for Membrane Antigens (GMMA), are being investigated as vaccines. Rapid methods are required for estimating the protein content for in-process assays during production. Since GMMA are complex biological structures containing lipid and polysaccharide as well as protein, protein determinations are...

Traditional colorimetric protein assays such as Biuret, Lowry, and modified Lowry (U-1988) are unsuitable for colored biological samples. Here we describe an improved Lowry protein assay (U-2012), which utilizes stable reagents and offers enhanced sensitivity over the U-1988 assay. U-2012 circumvents interference from colored pigments and other substances (for example sugars) bound to perchlori...

in present study different protein measurement methods are evaluated on the allergenic extract of chenopodium album pollen, which was previously prepared in our laboratory and applied in skin prick testing in comparison with a commer¬cial extract. the protein content of similar amounts of these two extracts which had caused similar skin reactions were measured with different protein assays such...

Biochemical analysis of proteins relies on accurate quantitation of protein concentration. This unit describes how to perform commonly used protein assays, e.g., Lowry, Bradford, BCA, and UV spectroscopic protein assays. The primary focus of the unit is assay selection, emphasizing sample and buffer compatibility. Protein assay standard curves and data processing fundamentals are discussed in d...

In 1989 the Committee on Apolipoproteins of the International Federation of Clinical Chemistry and the Centers for Disease Control conducted an international survey of total-protein measurements of isolated low-density lipoproteins (LDL) and delipidated high-density lipoproteins (HDL), and of their relationships to the National Institute of Standards and Technology (NIST) bovine serum albumin (...