Recombinant protein production in Pichia pastoris is based on alcohol oxidase promoterswhich are regulated by methanol. However, the use of methanol has several disadvantages,which is why current trends in bioprocess development with Pichia pastoris (P. pastoris) arefocusing on methanol mixed feeding strategies. This work aimed to develop a new experimentalmethod and compare the effect of vario...

Recombinant protein production in Pichia pastoris is based on alcohol oxidase promoterswhich are regulated by methanol. However, the use of methanol has several disadvantages,which is why current trends in bioprocess development with Pichia pastoris (P. pastoris) arefocusing on methanol mixed feeding strategies. This work aimed to develop a new experimentalmethod and compare the effect of vario...

Fermentation strategies for recombinant protein production in Pichia pastoris have been investigated and are reviewed here. Characteristics of the expression system, such as phenotypes and carbon utilization, are summarized. Recently reported results such as growth model establishment, application of a methanol sensor, optimization of substrate feeding strategy, DOstat controller design, mixed ...

The objective of this study was to assess whether and how coping strategies affect the welfare of African catfish Clarias gariepinus housed at low and high densities. Group composition influenced feed intake; re-active groups (comprised of 100% re-active fish) had a lower specific growth rate (G) and feed intake and a higher feed conversion ratio (R(FC)) than pro-active groups. Furthermore, re-...

In Denmark, it was agreed to lower the Salmonella prevalence in pork to 1.2% before the end of 2006. The current control did not seem to be sufficient to attain this goal. Therefore, four alternatives to the existing Danish control strategy for Salmonella in pork were compared in a cost-benefit analysis: (1) hot-water decontamination of all pigs at slaughter, (2) sanitary slaughter of pigs from...

This paper presents an approach to analytically determine the most energy efficient toolpath strategy in mechanical machining. This was achieved by evaluating the electrical energy requirement of the NC codes generated for the zag, zigzag, and rectangular contour toolpath strategies. The analytical method was validated by performing pocket milling on AISI 1018 steel with the considered toolpath...

thirty six male chall lambs (four-month old and 26  ±1.5 kg body weight) were randomly assigned into four treatments (nine lambs per treatment) to study the effect of powerfeed® (a commercial mixture made from wheat processing by-product and wheat bran) on weight gain, feed intake, feed conversion ratio, serum sodium and potassium concentration and urine ph. lambs in control group were fed with...

Thirty six male Chall lambs (four-month old and 26  ±1.5 kg body weight) were randomly assigned into four treatments (nine lambs per treatment) to study the effect of Powerfeed® (a commercial mixture made from wheat processing by-product and wheat bran) on weight gain, feed intake, feed conversion ratio, serum sodium and potassium concentration and urine pH. Lambs in control group were fed with...