The inducible Mar phenotype of Escherichia coli is associated with increased tolerance to multiple hydrophobic antibiotics as well as some highly hydrophobic organic solvents such as cyclohexane, mediated mainly through the AcrAB/TolC efflux system. The influence of water miscible alcohols ethanol and 1-propanol on a Mar constitutive mutant and a mar deletion mutant of E. coli K-12, as well as ...

Estimation of radical scavenging capacity of lipophilic antioxidants by electron spin resonance (ESR) in vitro is a challenging issue due to their poor solubility in aqueous radical generating and measuring systems. Water-miscible organic solvents are used for this purpose. A novel radical trapping agent, 5-(2,2-dimethyl-1,3-propoxy cyclophosphoryl)-5-methyl-1-pyrroline N-oxide (CYPMPO), that h...

Background: D-Phenylglycine aminotransferase (D-PhgAT) is highly beneficial in pharmaceutical biotechnology. Like many other enzymes, D-PhgAT suffers from low stability under harsh processing conditions, poor solubility of substrate, products and occasional microbial contamination. Incorporation of miscible organic solvents into the enzyme’s reaction is considered as a solution...

Water-miscible organic co-solvents can be used in DNA-based catalytic asymmetric reactions at appreciable concentration without a negative effect on enantioselectivity. While the rate of the copper(II) Diels-Alder reaction is affected negatively by the presence of organic co-solvents, the copper(II) catalyzed Michael addition and Friedel-Crafts alkylation reaction are significantly faster. Addi...

We propose a method for the modification of surfaces of microchannels in chips fabricated in polycarbonate (PC) that makes the devices resistant to a range of organic solvents. Coating of PC with branched polyethyleneimine (BPEI) with the use of trimethylpropane triglycidyl ether (TTE) as a linker renders the devices resistant to toluene, benzene, acetonitrile, tetrahydrofuran, dioxane and ethy...

The rate of reduction of 2,6-dichlorobenzenoneindophenol by xanthine oxidase plus its aldehydic or purine substrates is strikingly stimulated by the admixture of water-miscible organic solvents. In addition to acting as the electron acceptor in these assays, 2,6-dichlorobenzenoneindophenol is found to be a potent competitive inhibitor of xanthine oxidase with a K, of 1 X 10 6 M. The leuco form ...

in order to isolate novel organic solvent-tolerant (OST) lipases, a metagenomic library was built using DNA derived from a temperate forest soil sample. A two-step activity-based screening allowed the isolation of a lipolytic clone active in the presence of organic solvents. Sequencing of the plasmid pRBest recovered from the positive clone revealed the presence of a putative lipase/esterase en...

The catalytic activity of cytochrome P450 enzymes is known to be affected by presence of organic solvents in in vitro assays. However, these effects tend to be variable and depend on the substrate and CYP450 isoform in question. In the present study, we have investigated effect of ten water miscible organic solvents (methanol, ethanol, propanol, isopropanol, acetone, acetonitrile, dimethylsulph...

-A variety of water-miscible organic solvents caused profound activation of the homogeneous juvenile hormone esterase from Manduca sexta. Much lower activation was observed with other species. Methods for the handling of solutions of juvenile hormone are presented. The implications of solvent activation of juvenile hormone esterase activity are discussed, especially as they relate to analysis o...