two nuclear microsatellite dna loci along with partial sequences of mitochondrial dna trna pro -d-loop region were analyzed to assess the genetic diversity and population genetic structure of spottybelly greenling ( hexagrammos agrammus ). a total of 85 individuals were investigated from two different locations at the east and the south coasts of korea which are imwonjin (im) and tongyeong (tn)...

Objective(s): Variation in microsatellite sequences that are dispersed in the genome has been linked to a deficiency in cellular mismatch repair system and defects in several genes of this system are involved in carcinogenesis. Our aim in this study was to illustrate microsatellite DNA alteration in esophageal cancer. Materials and Methods: DNA was extracted from formalin fixed paraffin embedde...

introduction: recently, the incidence of fungal infections dramatically increased with an appearance of many novel species due to different criteria. therefore, several molecular methods have been established for identification of these agents which cause human and animal disorder based on genomic dna. among these methods, rapd-pcr technique is a powerful discriminative method based on amplifie...

objective(s): variation in microsatellite sequences that are dispersed in the genome has been linked to a deficiency in cellular mismatch repair system and defects in several genes of this system are involved in carcinogenesis. our aim in this study was to illustrate microsatellite dna alteration in esophageal cancer. materials and methods: dna was extracted from formalin fixed paraffin embedde...

the aim of the present study was the genetic analysis of the dniester population of sterlet acipenser ruthenus and comparison of it to five other sterlet populations, in order to develop a population recovery plan. the genetic analysis of six sterlet populations from eurasian rivers (dniester, dnieper, danube, volga, kama and ob) was carried out using microsatellite dna markers. the genetic var...

Objective(s): Genetic analysis of two consanguineous Pakistani families with localized autosomal recessive hypotrichosis was performed with the goal to establish genotype-phenotype correlation. Materials and Methods: Genomic DNA extraction had been done from peripheral blood samples. Extracted DNA was then subjected to PCR (polymerase chain reaction) for amplification. Linkage analysis was perf...

PURPOSE Tumor cells may release DNA into circulation, which is subsequently carried as free DNA and enriched in blood and urine. The detection of tumors by microsatellite analysis of free DNA offers a possibility to establish a minimally invasive method for the detection of bladder cancer. EXPERIMENTAL DESIGN We performed microsatellite analysis of free DNA of urine, serum, and plasma in comp...

This study aimed to estimate microsatellite based genetic diversity in two lines (the selected RIRS and control line RIRC) of Rhode Island Red (RIR) chicken. Genomic DNA of 24 randomly selected birds maintained at Central Avian Research Institute (India) and 24 microsatellite markers were used. Microsatellite alleles were determined on 6% urea-PAGE, recorded using GelDoc system and the samples ...

Somatic DNA alterations are known to occur in atherosclerotic carotid artery lesions; however, their significance is unknown. The accumulation of microsatellite mutations in coding DNA regions may reflect a deficiency of the DNA mismatch repair (MMR) system. Alternatively, accumulation of these coding microsatellite mutations may indicate that they contribute to the pathology. To discriminate b...

 Population genetic structure of Vimba vimba persa was investigated using microsatellite markers from 40 regions along the Iranian coastline of the Southern Caspian Sea (Anzali lagoon and Havigh River in Gilan province, BabolRoud River in Mazandaran province and GorganRoud River in Golestan province). Genomic DNA from 121 specimens was extracted from fin tissue by the Phenol-Chlorophorm method ...