We report herein the structuring of single-stranded thymine-rich DNA sequences into peptide-DNA hairpin triplex structures via designed melamine-thymine nucleobase recognition. Melamine-displaying α-peptides were synthesized with the general form (EM*)n, where M* denotes a lysine residue side chain derivatized with melamine, a bifacial hydrogen bond complement for thymine. We have found that (E...

Coordination of copper (I) and (II) salts to melamine (Mel) produces [CuX(Mel)] [X = Cl (5a), Br (5b), I (5c), NO3 (5d)], [CuX2(Mel)2] Æ S [X = Cl, S = H2O (6a); X = Cl, S = 2 MeOH (6b); X = Br, S = 1/2H2O (6c); X = OAc, S = none (6d)], and [Cu(OAc)2(Mel)] (7). The chemical identity of the complexes has been confirmed by elemental analysis, X-ray powder diffraction, thermogravimetry, and infrar...

A combination of molecular mechanics (MM), electron paramagnetic resonance spectroscopy (EPR), and spectra simulation (MM-EPR) has been used to determine the solution structures of di- and trinuclear copper(II) complexes of melamine-based oligomacrocyclic ligands. The spin Hamiltonian parameters of the mononuclear, melamine-appended macrocyclic ligand copper(II) complex have been determined by ...

Detection of melamine has been developed by employing oxidized polycrystalline gold electrode (poly GE). The poly GE was directly utilized for the detection of melamine using differential pulse voltammetry (DPV) and impedimetry. Poly GE successfully showed the oxidation peak for melamine adsorption at 1.1 V is purely based on the detection of adsorption signals of melamine at the electrode surf...

The 2008 Chinese milk scandal caused by the adulteration of melamine encouraged the public to pay attention to melamine detection in milk products and other food stuffs. To allow simple and rapid detection of melamine, we previously isolated an 88 nt melamine aptamer (called Rd29C33) using the structure-switching SELEX. However, this 88 nt oligonucleotide is costly to synthesize, and may also c...

Melamine poisoning has become widely publicized after a recent occurrence of renal injury in infants and children exposed to melamine-tainted milk in China. This renal damage is believed to result from kidney stones formed from melamine and uric acid or from melamine and its cocrystallizing chemical derivative, cyanuric acid. However, the composition of the stones and the mechanism by which the...

One of the key concerns of the people and the government of China is food safety. After the use of melamine as an additive was reported in 2008, melamine detection was required in milk. The current methods to detect and confirm melamine are mainly HPLC and HPLC-MS/MS, but the pre-treatment processes include some tedious steps making them time-consuming and environmentally unfriendly. In contras...

Recent cases of adulteration with melamine have led to the need for rapid and reliable screening methods. To meet this need, commercial enzyme-linked immunosorbent assay (ELISA) test kits for the detection of triazines were evaluated. The recently released Melamine Plate kit (Abraxis, Warminster, Pa.) displayed a limit of detection of 9 ng/ml for melamine in phosphate-buffered saline (PBS) and ...

Melamine is a nitrogen heterocyclic triazine compound which is widely used as an industrial chemical. Although melamine is not considered to be acutely toxic with a high LD50 in animals, food contaminated with melamine expose risks to the human health. Melamine has been reported to be responsible for the renal impairment in mammals, its toxicity on the reproductive system, however, has not been...

This article describes reactions of the trivalent melamine derivatives C6H3-1,3,5-[CONHC6H4-3-N(CH2C6H44-C(CHi)3)COC6H3-2-(NHCrN3(NH2XNHCH2CH2C(CH3)3))-5-Brl, (hubM3) and (CH3),COC(O)NHCICH2O(CHz)rSCH2C(O)NHC6H4-2-NHC3N3(NH2)(NH(CH2)2C(CH3)r)1, (trisMr) with the bivalent isocyanurate derivatives CuHzl ,5.[CH(CH)2|z-2,4.[cH,ff i(o)]z(benzCA,)andc4o-2'5-[cH(cH3)2]?-3'4-[cH,ff i6[ NHC(O)12 (furanC...