Background Oocyte maturity includes nuclear and cytoplasmic maturity, both of which are important for embryo fertilization. The development of oocyte is not limited to the period of follicular growth, and starts from the embryonic period and continues throughout life. In this study, for the purpose of evaluating the effect of the FSH hormone on the expression of genes, GEO access codes for this...

background: linoleic acid (la) is a polyunsaturated fatty acid present in high concentrations in follicular fluid, when added to maturation culture media, it affects oocyte competence.objective: in the present study, we investigated effect of linoleic acid supplementation on in vitro maturation, embryo development and apoptotic related gene expression in ovinematerials and methods: the experime...

Background: Vitrification of oocytes is a fast-freezing technique, which may affect the quality of the human oocyte, and consequently affects the embryo development, pregnancy and birth. The aim of the current study was to investigate the consequence of in-vitro vitrification on maturation status of immature human oocytes, additionally, expression levels of stress, and apoptosis related genes. ...

objective: nutrients and antioxidants in the medium of immature oocyte have a profound effect on maturation, fertilization and development of resulting embryos. in this study the effects of melatonin as an antioxidant agent on maturation, glutathione level and expression of high mobility group box-1 (hmgb1) gene were evaluated in immature oocytes of mice stained with brilliant cresyl blue (bcb)...

background: ammonium is produced in culture medium due to amino acids degradation and has adverse effect on in vitro culture of embryo. in the current study, the purpose was to evaluate the effects of ammuniom chloride (ac) on in vitro oocyte maturation rate and early embryo development in the sheep and its effect on the expression of bcl-2. methods: in vitro maturation (ivm) was performed in t...

Background In vitro maturation (IVM) of human oocytes has acquired increasing attention in infertility treatment with great promise. This technique is an alternative conventional in vitro fertilization-embryo transfer (IVF-ET), and can be reduced the side effects of gonadotropin stimulation such as ovarian hyperstimulation (OHSS). Oocyte maturation is a complex process including cytoplasmic and...

Background Follicle stimulating hormone (FSH)- a hetrodimeric glycoprotein- is secreted by pituitary gland. This hormone stimulates growth and maturation of the follicles in females and sperms in male. Up to now, glycoprotein hormones such as FSH have produced in different cell lines. Among of the mammalian expression systems, the Chinese hamster ovary cells (CHO) have taken into consideration ...

Background: Androgens are critical steroid hormones in progression of spermatogenesis process and determine the male phenotype that their actions are mediated by the androgen receptor (AR), a member of the nuclear receptor superfamily. In the Androgen receptor, transactivation domain encoded by exon 1, DNA binding domain encoded by exons 2 and 3, hinge region encoded by part of exon 4, and C-te...

Background We have previously reported the effect of Anthocyanin and cAMP modulators on maturation and apoptosis of sheep oocytes. In this study, we investigated the reduction capacity and apoptotic gene expression results at MII sheep oocytes. MaterialsAndMethods Good ovine Cumulus-oocyte complexes (COCs) were transferred to Pre-IVM medium, then to two type IVM mediums: 1) 0.1μg/ml Delphinidin...