background: fetal dna in maternal plasma and serum has been shown to be a useful material for prenatal fetal sex determination during early gestational ages. non-invasive prenatal diagnosis is now possible at 8th week of pregnancy, by maternal blood sample testing. objective: the purpose of this study was to evaluate two dna extraction methods from mother plasma and its routine clinical applica...

Background: Current prenatal diagnosis requires invasive testing which carries a 1-4% procedure-related-risk of miscarriage; hence, non-invasive techniques are desired. The recent demonstration of cell-free-fetal-DNA enriched from maternal plasma has opened new possibilities for non-invasive-prenatal-diagnosis of not only genetic-disorders such as β-thalassaemia and haemophilia but also chromos...

Background: Current prenatal diagnosis requires invasive testing which carries a 1-4% procedure-related-risk of miscarriage; hence, non-invasive techniques are desired. The recent demonstration of cell-free-fetal-DNA enriched from maternal plasma has opened new possibilities for non-invasive-prenatal-diagnosis of not only genetic-disorders such as β-thalassaemia and haemophilia but also chromos...

background: pre-eclampsia, a vascular disorder of pregnancy, is a leading cause of maternal morbidity as well as perinatal morbidity and mortality. the cause of pre-eclampsia remains largely unknown. accumulating evidences from clinical and epidemiological studies suggest that diffuse endothelial dysfunction, resulting from oxidative stress, plays an important role in the pathogenesis of pre-ec...

Background and Aims: New advances in the use of cell-free fetal DNA (cffDNA) in maternal plasma of pregnant women has provided the possibility of applying cffDNA in prenatal diagnosis as a non-invasive method. One of the applications of prenatal diagnosis is fetal gender determination. Early prenatal determination of fetal sex is required for pregnant women at risk of X-linked and some endocrin...

background: fetal dna has been found in maternal plasma. diagnosis of fetal gender using maternal plasma has been attempted in an effort to develop a new noninvasive method of prenatal diagnosis. methods: we extracted dna from plasma of 30 pregnant woman, three men, three non-pregnant women, and performed a sensitive y-pcr assay to detect circulating male fetal dna from women bearing male fetus...

The aim of the present study was to test the hypothesis that maternal intake of antioxidant vitamins is associated with maternal and cord plasma levels at delivery. Women were recruited in early pregnancy in Aberdeen Maternity Hospital and habitual diet during pregnancy was assessed by a food-frequency questionnaire mailed at 34 weeks gestation. Blood samples were taken at recruitment (n 1149) ...