A facile method for accurate detection of type 1 dengue virus (DV1) infection from complex biological mixtures, using type specific immunocapture coupled with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), was developed. The biological mixtures were treated with magnetic particles coated with a monoclonal antibody directly against type 1 dengue viru...

The isolation of circulating tumor cells (CTCs) from cancer patient blood is a technical challenge that is often addressed by microfluidic approaches. Two of the most prominent techniques for rare cancer cell separation, immunocapture and dielectrophoresis (DEP), are currently limited by a performance tradeoff between high efficiency and high purity. The development of a platform capable of the...

The capture of circulating tumor cells (CTCs) from cancer patient blood enables early clinical assessment as well as genetic and pharmacological evaluation of cancer and metastasis. Although there have been many microfluidic immunocapture and electrokinetic techniques developed for isolating rare cancer cells, these techniques are often limited by a capture performance tradeoff between high eff...

This research aimed to integrate an established signalquantification technique with immunocapture microneedle arrays (MNAs) improvebiomarker-based disease diagnostics in the skin. In this miniaturized sandwichenzyme-linked immunosorbent assay (ELISA) platform, capture antibody wasimmobilised onto surface of microneedles which, when inserted superficiallyinto porcine skin, captured endogenous im...

Background: Shigella is among the most important human pathogenic microorganisms, infecting both humans and nonhuman and causing clinically severe diarrhea. Shigella must be enriched before detection, which is time-consuming.Objectives: To develop a sensitive, rapid, and specifc method for Shigella detection.Materials and Methods: S...

Background: Salmonella can cause serious human gastroenteritis and is frequently isolated from various food samples. The cell culturing, immunoassay, and polymerase chain reactions (PCR) are the current methods to detect such pathogenic agents. However, these methods are time-consuming and labor-intensive, and thus unavailable for rapid-monitoring of Salmonella</em...

Detection of Mycobacterium paratuber culosis in feces is complicated by the slowdivision and the lipid composition of the cell wall of the bacterium. In this study, we compared a rapid and sensitive method for direct detection of M. paratuberculosis with anti-paratuberculosis chicken IgY-conjugated magnetic nanoparticles versus commercially available micro-sized paramagnetic beads. Tenfold seri...

Aiming at clinical studies of human diseases, antibody-assisted assays have been applied to biomarker discovery and toward a streamlined translation from patient profiling to assays supporting personalized treatments. In recent years, integrated strategies to couple and combine antibodies with mass spectrometry-based proteomic efforts have emerged, allowing for novel possibilities in basic and ...