نتایج جستجو برای: MTT formazan

تعداد نتایج: 15467  

2012
Hülya Şenöz

ÖZ E T Bu çalışmada tetrazolyum tuzları ve formazanların yapıları tanımlanmıştır. Ayrıca formazan-tetrazolyum sistemlerinin önemi ve kullanım alanları açıklanmıştır. Formazanların sahip oldukları conjugate p-system kaynaklanan tautomerleşme özellikleri ve geometrik izomerleri incelenmiştir. Bu bileşiklerin en yaygın kullanılan sentezleri ve yapılarının açıklanmasında önemli olan UV-visible, IR ...

Journal: :Investigative ophthalmology 1964
H E KAUFMAN J A CAPELLA J E ROBBINS

T, . etrazolium stains permit the histochemical demonstration of dehydrogenase enzymes in tissue sections. These stains are soluble compounds which act as hydrogen acceptors developing insoluble formazan precipitates in the reduced state. Blue tetrazolium was first used by DeBerardinis to study frozen corneal tissue sections and subsequently has been used by Kuwabara and Cogan, who studied the ...

Journal: :European journal of histochemistry : EJH 2007
Giacomo Diaz M Melis A Musin M Piludu M Piras A M Falchi

MTT (3-(4, 5-dimethyl-2-thiazolyl)-2, 5-dihphenyltetrazolium bromide) assay is a widely used method to assess cell viability and proliferation. MTT is readily taken up by cells and enzymatically reduced to formazan, a dark compound which accumulates in cytoplasmic granules. Formazan is later eliminated by the cell by a mechanisms often indicated as exocytosis, that produces characteristic needl...

Journal: :Cancer research 1991
D T Vistica P Skehan D Scudiero A Monks A Pittman M R Boyd

The hydrogen acceptor 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) is commonly utilized to estimate cellular viability in drug screening protocols. The present investigation was prompted, in part, by observations that reduction of MTT to its colored reaction product, MTT formazan, varied between cell lines and with culture age. A correlation was established between the D-g...

Journal: :The Analyst 2011
Ruiling Qi Mingwu Shen Xueyan Cao Rui Guo Xuejiao Tian Jianyong Yu Xiangyang Shi

One major method used to evaluate the biocompatibility of porous tissue engineering scaffolding materials is MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The MTT cell viability assay is based on the absorbance of the dissolved MTT formazan crystals formed in living cells, which is proportional to the number of viable cells. Due to the strong dye sorption capability ...

2015
B.M. van den Berg

The ability of sperm cells to develop colored formazan by reduction of MTT was used earlier to develop a spectrophotometric assay to determine the viability of sperm cells for several mammalian species. It was the objective of the present study to visualize microscopically the location of the formazan in boar sperm cells. The MTT staining process of boar sperm cells can be divided into a series...

2015
B. M. van den Berg

The ability of sperm cells to develop colored formazan by reduction of MTT was used earlier to develop a spectrophotometric assay to determine the viability of sperm cells for several mammalian species. It was the objective of the present study to visualize microscopically the location of the formazan in boar sperm cells. The MTT staining process of boar sperm cells can be divided into a series...

2006
David T. Vistica Philip Skehan Dominic Scudiero Anne Monks Angela Pittman Michael R. Boyd

The hydrogen acceptor 3-(4,5-dimethylthiazol-2-yl)-2,5 dipheny Itetrazolium bromide (Mil) is commonly utilized to estimate cellular viability in drug screening protocols. The present investigation was prompted, in part, by observations that reduction of MTT to its colored reaction product, MTT formazan, varied between cell lines and with culture age. A correlation was established between the D-...

Journal: :The Journal of Biophysical and Biochemical Cytology 1961
Alex B. Novikoff Woo-Yung Shin Joan Drucker

A comparison is made of the staining results obtained with Nitro-BT and MTT-Co(++) as acceptors when DPNH is the substrate in frozen sections of cold formol-calcium-fixed rat kidney (normal and following ligation of the blood vessels) and human liver containing lipofuscin granules. The kidney results are evaluated in terms of mitochondrial morphology seen after classical mitochondrial stains an...

Journal: :Journal of immunological methods 1986
F Denizot R Lang

A convenient way to estimate the number of viable cells growing in microtitre tray wells is to use a colorimetric assay and an automatic microplate scanning spectrophotometer. One such assay, developed by Mosmann, depends on the reduction by living cells of tetrazolium salt, MTT, to form a blue formazan product. However the original technique has several technical limitations, namely a less tha...

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