Purpose: To investigate the mechanism of microRNA-124 action on neuroblastoma apoptosis induced by sevoflurane.
 Methods: MiR-124 expression was assessed in a cell line (SMS-KAN) using quantitative reverse transcription polymerase chain reaction (qRT-PCR). The role miR-124 sevoflurane anesthesia-induced studied activity and analysis 3-(4, 5-dimethylthiazolyl-2-yl)-2-5 diphenyl tetrazolium ...

The MTT antibacterial assay is an important method in natural component discovery. Researchers can use the to quickly and efficiently evaluate activity of compounds. aim this research was find a reduce background colour bacterial cell interference when using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) study effect phytochemicals. This used NZ ‘Hass’ ripe avocado seed ext...

Primula auriculata (Tootia) is one of the most important local medicinal plants in Hamedan district, Iran. To investigate cytotoxicity and apoptosis induction of crude methanolic extract and different fraction of it, we compared several methods on HT-29 human colon Adenocarcinoma cells. Cancer cell proliferation was measured by 3-(4, 5‑dimethylthiazolyl)2, 5‑diphenyl‑tetrazolium bromide (MTT) a...

Primula auriculata (Tootia) is one of the most important local medicinal plants in Hamedan district, Iran. To investigate cytotoxicity and apoptosis induction of crude methanolic extract and different fraction of it we compared several methods on HT-29 human colon Adenocarcinoma cells. Cancer cell proliferation was measured by 3-(4, 5‑dimethylthiazolyl)-2, 5‑diphenyl‑tetrazolium bromide (MTT) a...

OBJECTIVE This study evaluated in vitro cell viability by the colorimetric MTT stands for 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay compared to image analysis by CellProfiler® software. MATERIALS AND METHODS Hepatoma (Hepa-1c1c7) and fibrobla...

primula auriculata (tootia) is one of the most important local medicinal plants in hamedan district, iran. to investigate cytotoxicity and apoptosis induction of crude methanolic extract and different fraction of it we compared several methods on ht-29 human colon adenocarcinoma cells. cancer cell proliferation was measured by 3-(4, 5‑dimethylthiazolyl)-2, 5‑diphenyl‑tetrazolium bromide (mtt) a...

Primula auriculata (Tootia) is one of the most important local medicinal plants in Hamedan district, Iran. To investigate cytotoxicity and apoptosis induction of crude methanolic extract and different fraction of it we compared several methods on HT-29 human colon Adenocarcinoma cells. Cancer cell proliferation was measured by 3-(4, 5‑dimethylthiazolyl)-2, 5‑diphenyl‑tetrazolium bromide (MTT) a...

the antioxidant and cytotoxic properties of four major parts of methanolic extracts of tephrosia purpurea including leaves, root, stem and seed were investigated and compared. in vitro antioxidant activity of t. purpurea extracts was evaluated using 1,1-diphenyl-2-picrylhydrazyl (dpph), ferric reducing antioxidant power (frap), reducing power assay and antihemolytic assay. in vitro cytotoxic ef...

Breast cancer is the most common cancer type among women all over the world. Chemotherapy is the use of anticancer medicines for treating cancer but it has many side effects and cells may become resistant to these chemical medicines. Therefore, finding new compounds of natural origin could be a promising solution to this problem. The aim of the current study was to evaluate anticancer activity ...

This paper describes a critical evaluation of a miniaturised colorimetric assay, using MTT (3-[4,5-dimethyl-thiazol-2-yl]-2,5-diphenyl-tetrazolium bromide) reduction, applied to protozoan viability testing. The toxic substances used were copper, zinc, Triton X-100 (a membrane surfactant) and cycloheximide (an inhibitor of the protein synthesis). The viability assay of the ciliate protozoan Tetr...