Microsomal triglyceride transfer protein (MTP) transfers lipids to apolipoprotein B (apoB) within the endoplasmic reticulum, a process that involves direct interactions between apoB and the large subunit of MTP. Recent studies with heterozygous MTP knockout mice have suggested that half-normal levels of MTP in the liver reduce apoB secretion. We hypothesized that reduced apoB secretion in the s...

RATIONALE Systemic inflammation has emerged as a key pathophysiological process that induces multiorgan injury and causes serious human diseases. Endothelium is critical in maintaining cellular and inflammatory homeostasis, controlling systemic inflammation, and progression of inflammatory diseases. We postulated that endothelium produces and releases endogenous soluble factors to modulate infl...

Microsomal triglyceride transfer protein (MTP) is required for the assembly and secretion of apolipoprotein (apo) B-containing lipoproteins. Previously, we demonstrated that the N-terminal 1,000 residues of apoB (apoB:1000) are necessary for the initiation of apoB-containing lipoprotein assembly in rat hepatoma McA-RH7777 cells and that these particles are phospholipid (PL) rich. To determine i...

This paper reports an investigation on the modulation of the mitochondrial permeability transition pore (MTP) by the membrane potential. Energized rat liver mitochondria loaded with a small Ca2+ pulse in sucrose medium supplemented with phosphate favor a high MTP "closed" probability because of the high membrane potential and therefore maintain a low permeability to sucrose. Upon depolarization...

The high silica Mn-substituted MFI metallosilicate catalyst with Si/Al molar ratio of 220 and Si/Mn molar ratio of 50 was successfully synthesized by hydrothermal method. The catalyst sample was appropriately characterized by XRD, FE-SEM, EDX and BET techniques. The Mn-substituted MFI metallosilicate has not been reported as the potential catalyst for the methanol to propylene (MTP) reaction. T...

Microsomal triglyceride transfer protein (MTP) is a unique lipid transfer protein essential for the assembly of triglyceride-rich lipoproteins by the liver and intestine. Previous studies in mice identified a splice variant of MTP with an alternate first exon. Splice variants of human MTP have not been reported. Using PCR approaches we have identified two splice variants in human tissues, which...

The exergetic analysis is a tool that has been used successfully in many studies aiming a more rational energy consumption to reduce the cost of processes. With this analysis, it is possible to perform an evaluation of the overall process, locating and quantifying the degradation of exergy. This paper applies exergy approach for analyzing the heat exchanger network design and refrigeration of M...

Nanosheets of MFI zeolite were successfully synthesized by hydrothermal method and were properly characterized by XRD, FE-SEM, EDX-dot mapping, TEM, FT-IR, N2 adsorption/desorption, NH3-TPD, ICP-AES and TGA techniques. The characterizations of MFI zeolite nanosheets suitably confirmed well synthesized MFI nanostructure, superior specific surface area (695.96m2.g-1) and great mesopores volume. C...

Infective larvae (L3) of nematodes secrete macromolecules that are critical to infection and establishment of the parasite in the host. The dog hookworm Ancylostoma caninum secretes an astacin-like metalloprotease, Ac-MTP-1, upon activation in vitro with host serum. Recombinant Ac-MTP-1 was expressed in the baculovirus/insect cell system as a secreted protein and was purified from culture mediu...

Microsomal triglyceride transfer protein (MTP) is essential for the assembly of triglyceride-rich apolipoprotein B-containing lipoproteins. Previous studies in our laboratory identified a novel splice variant of MTP in mice that we named MTP-B. MTP-B has a unique first exon (1B) located 2.7 kB upstream of the first exon (1A) for canonical MTP (MTP-A). The two mature isoforms, though nearly iden...